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Molecule Parameter List for PA.Ca.PLCbeta | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by  color. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Statistics | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PA.Ca.PLCbeta participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction |
| No. of occurrences | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Accession and Pathway Details |
| Accession Name | Accession No. | Accession Type | Pathway Link |
| PLCbeta1 | 13 | Pathway | PLCbeta |
| This is a model of PLCbeta1 in the bovine brain membranes as stimulated by Ca, Gq and PA. The primary data sources are Irene Litosch Biochemistry (2000) 39(26):7736-43, Sternweis PC et al. Philos Trans R Soc Lond B Biol Sci. (1992) 336(1276):35-41 and Smrcka AV et al. Science (1991) 251(4995):804-7. | |||
PA.Ca.PLCbeta acting as a Molecule in PLCbeta1 Network
| Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | |
| PA.Ca.PLCbeta | PLCbeta1 Accession No. : 13 | PLCbeta Pathway No. : 67 | 0 | 0.0016667 | No | |
| 6 Nov 2001 This is the active enzyme complex formed by binding of PA with Ca.PLCbeta.All the activity of PA is possible only in the presence of Calcium bound enzyme i.e., Ca.PLCbeta and resulted in dose response curves for the activity obtained by I. Litosch., Biochem., 2000, 39, 7736-7743. | ||||||
PA.Ca.PLCbeta acting as an Enzyme in PLCbeta1 Network
| Enzyme Molecule / Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents |
| PA.Ca.PLCbeta / PA.Ca.PLCbeta | PLCbeta1 Accession No. : 13 | PLCbeta Pathway No. : 67 | 10 | 38 | 4 | Classical Michaelis-Menten V = Etot.S.Kcat/Km+S | Substrate PIP2 Product DAG IP3 |
| 3 Nov 2001 The Km and Vmax were fixed relatively to the Km and Vmax values of Ca.PLCbeta and Gq.Ca.PLCbeta which were obtained from the works of Sternweis et al and Smrcka et al where the Km varied between 5 and 20uM and Vmax between 7 and 23 umol/min/mg depending on the presence and absence of Gq. | |||||||
PA.Ca.PLCbeta acting as a Product in a reaction in PLCbeta1 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents |
ta | PLCbeta1 Accession No. : 13 | PLCbeta Pathway No. : 67 | 1 (uM^-1 s^-1) | 8 (s^-1) | Kd(bf) = 8(uM) | - | Substrate Ca.PLCbeta PA Product PA.Ca.PLCbeta |
| 6 Nov 2001 The Kf and Kb values were fixed according to the dose response curves obtained from I. Litosch, Biochem., 2000, 39, 7736-7743. | |||||||