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Molecule Parameter List for PLC-Ca-Gq | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Osc_Ca_
IP3metabolism | 32 | Network |
MIPP, CaMKII, CaM,
PKC, IP3-3K, Gq,
PLCbeta, 134_dephos, 145_dephos,
IP4-system, IHP-system, 1345_dephos,
CaRegulation, Othmer-Tang-model | | This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. |
PLC-Ca-Gq acting as a Molecule in Osc_Ca_IP3metabolism Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | PLC-Ca-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 0 | 1000 | No | | This should really be labelled PLC-Ca-GTP.Gq_alpha This is the most active form of the enzyme. |
PLC-Ca-Gq acting as an Enzyme in Osc_Ca_IP3metabolism Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | PLC-Ca-Gq /
PLCb-Ca-Gq
| Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 4.99994 | 160 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | Km: Sternweis et al, Phil Trans R Soc Lond 1992 Vmax: Smrcka et al, Science 1991 |
PLC-Ca-Gq acting as a Substrate in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | Inact-PLC-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 1.6667
(s^-1) | 0
(uM^-1 s^-1) | - | - | Substrate
PLC-Ca-Gq
Product
G*GDP
PLC-Ca
| | Rate of 100/min to account for GAP activity of PLC: Biddlecome et al, JBC, 271, 14, 7999-8007, 1996 |
PLC-Ca-Gq acting as a Product in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | PLC-Gq-bind-Ca | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 30
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.0333(uM) | - | Substrate
Ca
PLC-Gq
Product
PLC-Ca-Gq
| | | this step has a high affinity for Ca, from Smrcka et al. 0.1uM so kf /kb = 1/6e4 = 1.666e-5:1. See the Act-PLC-by-Gq reaction. Raised kf to 5e-5 based on match to conc-eff curves from Smrcka et al. | | 2 | Act-PLC-by-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 25.2
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.0397(uM) | - | Substrate
G*GTP
PLC-Ca
Product
PLC-Ca-Gq
| | | Affinity for Gq is > 20 nM (Smrcka et al Science251 804-807 1991) so [Gq].kf = kb so 40nM * 6e5 = kb/kf = 24e3 so kf = 4.2e-5, kb =1 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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