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Molecule Parameter List for nonhemebind_int | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | cGMP_regulation | 34 | Network |
Shared_Object_cGMP_regulation, GC, PDE | Though Corbin JD. et al. Eur J Biochem. (2000) 267(9):2760-7 has been mentioned in the citation, this model has been made with inputs from different literature sources, each of which has been mentioned in the notes sections. This model features hydrolysis of cGMP by bovine PDE, phosphorylation of PDE by bovine lung PKG, and activation of bovine lung PKG by cGMP binding. These mechanisms are known to be involved in cGMP level regulation. Rates have been used from different sources and the model has been tested based on Corbin JD. et al., since their work involved measuring the PDE phosphorylation and PDE activity.
On replicating Figures 2, 3 and 4 from their paper, there is approx 30% difference in results but the qualitative shape of the curves is very similar. This might be due to the fact that the Vmax values were used from different literature sources. This might lead to the discrepancy in the numbers in this model. The values shown in this model are near estimated physiological levels.In order to replicate the Figures more closely, we have run additional simulations with concentration terms changed so as to replicate the experimental conditions exactly. |
nonhemebind_int acting as a Molecule in cGMP_regulation Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | nonhemebind_int | cGMP_regulation
Accession No. : 34 | GC
Pathway No. : 177 | 0 | 0.0016667 | No | | here the new NO has bound to the unidentified non-heme site on the 6-coordinate complex, and this will convert to the 5-coordinate complex. this binding of a new NO to the non-heme site on the 6-coordinate complex is characteristic of the slow binding process of NO to sGC. |
nonhemebind_int acting as a Substrate in a reaction in cGMP_regulation Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | act_sGCslow | cGMP_regulation
Accession No. : 34 | GC
Pathway No. : 177 | 1.6
(s^-1) | 0.02
(s^-1) | Keq = 0.0125(uM) | 0.617sec | Substrate
nonhemebind_int
Product
NO2.sGC_5coord
| | Rates used directly from Stone and Marletta,1996, Biochemistry, 35(4):1093-1099. |
nonhemebind_int acting as a Product in a reaction in cGMP_regulation Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | NObindnonheme | cGMP_regulation
Accession No. : 34 | GC
Pathway No. : 177 | 5
(uM^-1 s^-1) | 25
(s^-1) | Kd(bf) = 5(uM) | - | Substrate
NO
NO.sGC6coord
Product
nonhemebind_int
| | This step is the one that differs from the fast reaction scheme, as reported by Stone and Marletta. Here the reaction is dependent upon the binding of NO to an unidentified non-heme site on the protein. Rates used directly from Stone and Marletta,1996, Biochemistry, 35(4):1093-1099. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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