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Molecule Parameter List for Shc*.Sos.Grb2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Shc*.Sos.Grb2 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010001

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • mkp1_feedback_
    effects
    		• 4Network
    Shared_Object_mkp1_feedback_effects,  Sos,  PKC,  
    MAPK,  PLA2,  Ras,  
    PDGFR 
    This is a network involving the MAPK-PKC feedback loop with input from the PDGFR in the synapse. The distinctive feature of this model is that it includes MKP-1 induction by MAPK, and the consequent inhibitory regulation of MAPK and the feedback loop. Lots of interesting dynamics arise from this. This link provides supplementary material for the paper Bhalla US et al. Science (2002) 297(5583):1018-23. In the form of several example simulations and demos for the figures in the paper.

    Shc*.Sos.Grb2 acting as a Molecule in      mkp1_feedback_effects Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    Shc*.Sos.Grb2
  • mkp1_feedback_
    effects
    Accession No. : 4
  • Shared_Object_
    mkp1_feedback_
    effects
    Pathway No. : 32
    		• 01000No
    This three-way complex is one of the main GEFs for activating Ras.

    Shc*.Sos.Grb2 acting as an Enzyme in      mkp1_feedback_effects Network
    Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    Shc*.Sos.Grb2 /
    Sos.Ras_GEF
  • mkp1_feedback_
    effects
    Accession No. : 4
  • Shared_Object_
    mkp1_feedback_
    effects
    Pathway No. : 32
    		• 0.5050510.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
    Rates are from Orita S, Kaibuchi K, Kuroda S, Shimizu K, Nakanishi H, Takai Y (1993) J Biol Chem. 268(34):25542-25546

    Shc*.Sos.Grb2 acting as a Product in a reaction in      mkp1_feedback_effects Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • Shc_bind_
    Sos.Grb2
  • mkp1_feedback_
    effects
    Accession No. : 4
    		• Sos
    Pathway No. : 33    		0.5
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.2(uM)-Substrate
    SHC*
    Sos.Grb2

    Product
    Shc*.Sos.Grb2
    Sasaoka et al JBC 269:51 pp 32621 1994, table on pg 32623 indicates that this pathway accounts for about 50% of the GEF activation. (88% - 39%). Error is large, about 20%. Fig 1 is most useful in constraining rates. Chook et al JBC 271:48 pp 30472, 1996 say that the Kd is 0.2 uM for Shc binding to EGFR. The Kd for Grb direct binding is 0.7, so we'll ignore it.


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
    This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.