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Molecule Parameter List for inact-GEF

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
inact-GEF participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001001

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • mkp1_feedback_
    effects
    		• 4Network
    Shared_Object_mkp1_feedback_effects,  Sos,  PKC,  
    MAPK,  PLA2,  Ras,  
    PDGFR 
    This is a network involving the MAPK-PKC feedback loop with input from the PDGFR in the synapse. The distinctive feature of this model is that it includes MKP-1 induction by MAPK, and the consequent inhibitory regulation of MAPK and the feedback loop. Lots of interesting dynamics arise from this. This link provides supplementary material for the paper Bhalla US et al. Science (2002) 297(5583):1018-23. In the form of several example simulations and demos for the figures in the paper.

    inact-GEF acting as a Molecule in      mkp1_feedback_effects Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    inact-GEF
  • mkp1_feedback_
    effects
    Accession No. : 4
    		• Ras
    Pathway No. : 37    		0.11000No
    This is the amount of inactive GEF available to the system. The value is the same as the estimated amount of SoS, though I treat it here as a different pool. Probably several molecules can function as GEFs and this is a simplification. Orita et al JBC 268(34):25542-25546 Gulbins et al 1994 Mol Cell Biol 14(2):906-913

    inact-GEF acting as a Substrate for an Enzyme in      mkp1_feedback_effects Network
    Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PKC-active  /
    PKC-act-GEF
  • mkp1_feedback_
    effects
    Accession No. : 4
  • Shared_Object_
    mkp1_feedback_
    effects
    Pathway No. : 32
    		• 66.666744explicit E-S complexSubstrate
    inact-GEF

    Product
    GEF*
    Rate constants are generic PKC rates. See Chen SJ, Klann E, Gower MC, Powell CM, Sessoms JS, Sweatt JD. (1993) Biochemistry 32(4):1032-9. This reaction activates GEF. Gives >= 2X stim of ras, and a 2X stim of MAPK over amount from direct phosph of c-raf. Note that it is a push-pull reaction, and also get effect through phosph and inact of GAPs.

    inact-GEF acting as a Product in a reaction in      mkp1_feedback_effects Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    dephosph-GEF
  • mkp1_feedback_
    effects
    Accession No. : 4
    		• Ras
    Pathway No. : 37    		0.1
    (s^-1)    		0
    (s^-1)    		--Substrate
    GEF*

    Product
    inact-GEF
    This rate is based on the known ratio of GDP-Ras to GTP-Ras. Basal: Ras.GTP = 7% Stimulated 15% Time course is within 10 min, probably much faster as not all early data points are there. See Gibbs et al JBC 265(33):20437-20422


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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