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Molecule Parameter List for GTP.Ga | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | PKA_2003 | 47 | Network |
Shared_Object_PKA_2003, PKA, AC,
Gs | | This model consists of receptor-ligand interaction, G-protein activation, Adenylyl cyclase mediated formation of cAMP and activation of PKA in the neuron. Demonstration programs using this model described in Bhalla US. (2004) Biophys J. 87(2):733-44 to generate a dose-response curve using stochastic calculations are available here. |
GTP.Ga acting as a Molecule in PKA_2003 Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | GTP.Ga | PKA_2003
Accession No. : 47 | Shared_Object_
PKA_2003
Pathway No. : 195 | 0 | 1000 | No | | Activated G protein. Supposed to be 15-40% of total Gs when max stim. |
GTP.Ga acting as a Substrate in a reaction in PKA_2003 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | Gs-bind-AC | PKA_2003
Accession No. : 47 | AC
Pathway No. : 197 | 499.998
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.002(uM) | - | Substrate
AC
GTP.Ga
Product
Gs.AC
| | | Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer Jacobowitz thesis (Mount Sinai 1995) data indicates it is more like 2 nM. | | 2 | GTPase | PKA_2003
Accession No. : 47 | Gs
Pathway No. : 198 | 0.0667
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP.Ga
Product
GDP.Ga
| | | From Brandt and Ross JBC 261(4):1656-1664, rate is 4/min and is agonist independent. I treat this as irreversible. |
GTP.Ga acting as a Product in a reaction in PKA_2003 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | Activate-Gs | PKA_2003
Accession No. : 47 | Gs
Pathway No. : 198 | 0.025
(s^-1) | 0
(uM^-2 s^-1) | - | - | Substrate
L.R.GDP.Gabc
Product
GTP.Ga
Gbg
L.R
| | This step combines several stages in GTP.Galpha release. From Berstein et al activation is at .35 - 0.7/min From Fay et al Biochem 30 5066-5075 1991 kf = .01/sec. From Brandt and Ross JBC 261(4):1656-1664 (1986) and Ransan et al Biochem J 283(2):519-524 (1992) rates around 2.5/min to 1.5/min are better. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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