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Molecule Parameter List for Sos*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Sos* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000120

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
EGFR_MAPK58Network
Shared_Object_EGFR_MAPK,  MAPK,  Ras,  
EGFR,  Sos 
Model of MAPK activation by EGFR in the synapse. Demonstration programs using this model are available here.
Primary citation:Bhalla US. Biophys J. (2004) 87(2):745-53.

Sos* acting as a Molecule in  EGFR_MAPK Network
NameAccession NamePathway NameInitial Conc.
(uM)		Volume
(fL)		Buffered
Sos*EGFR_MAPK
Accession No. : 58		Sos
Pathway No. : 233		01000No

Sos* acting as a Product of an Enzyme in  EGFR_MAPK Network
Enzyme Molecule /
Enzyme Activity		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
MAPK*  /
phosph_Sos		EGFR_MAPK
Accession No. : 58
  • Shared_Object_
    EGFR_MAPK
    Pathway No. : 229
    		• 2.5641104explicit E-S complexSubstrate
    Sos

    Product
    Sos*
    See Porfiri and McCormick JBC 271:10 pp5871 1996 for the existence of this step. We'll take the rates from the ones used for the phosph of Raf by MAPK. Sep 17 1997: The transient activation curve matches better with k1 up by 10 x.

    Sos* acting as a Substrate in a reaction in      EGFR_MAPK Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Grb2_bind_Sos*EGFR_MAPK
    Accession No. : 58    		Sos
    Pathway No. : 233    		0.025
    (uM^-1 s^-1)    		0.0168
    (s^-1)    		Kd(bf) = 0.672(uM)-Substrate
    Grb2
    Sos*

    Product
    Sos*.Grb2
      Same rates as Grb2_bind_Sos: Porfiri and McCormick JBC 271:10 pp 5871 1996 show that the binding is not affected by the phosph.
    2dephosph_SosEGFR_MAPK
    Accession No. : 58    		Sos
    Pathway No. : 233    		0.001
    (s^-1)    		0
    (s^-1)    		--Substrate
    Sos*

    Product
    Sos
      The only clue I have to these rates is from the time courses of the EGF activation, which is around 1 to 5 min. The dephosph would be expected to be of the same order, perhaps a bit longer. Lets use 0.002 which is about 8 min. Sep 17: The transient activation curve matches better with kf = 0.001


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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