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Molecule Parameter List for Im-nNOS-L-Arginine | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | L-Arginine | 72 | Pathway |
L-Arginine | This model was taken from the Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. This model shows kinetic binding of L-Arginine to neuronal nitric oxide synthase.
Model shows the substrate (L-Arginine) binding to nNOS in a two-step reversible fashion. First there is rapid binding equilibrium between Im-nNOS and L-Arginine to form an intermediate that contains bound imidazole and L-Arginine. This is followed by a slower conformational change in the Im-enzyme-substrate complex that is associated with release of bound imidazole and generation of a modified enzyme-substrate complex which is detected due to spectral change. Replicates Figure 4 (right panel).
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Im-nNOS-L-Arginine acting as a Molecule in L-Arginine Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | Im-nNOS-L-Arginine | L-Arginine
Accession No. : 72 | L-Arginine
Pathway No. : 308 | 0 | 0.0016667 | No | | An intermediate formed due to rapid binding of L-Arginine to Imidazole bound neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
Im-nNOS-L-Arginine acting as a Substrate in a reaction in L-Arginine Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | Imidazole_
release | L-Arginine
Accession No. : 72 | L-Arginine
Pathway No. : 308 | 81
(s^-1) | 0.45
(uM^-1 s^-1) | Kd(cb) = 0.0056(uM) | - | Substrate
Im-nNOS-L-Argini
ne
Product
Imidazole
nNOS-L-Arginine
| | Release of Imidazole from complex of L-Arginine and Imidazole bound rat brain neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
Im-nNOS-L-Arginine acting as a Product in a reaction in L-Arginine Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | Arginine_
binding | L-Arginine
Accession No. : 72 | L-Arginine
Pathway No. : 308 | 250
(uM^-1 s^-1) | 125000
(s^-1) | Kd(bf) = 500(uM) | - | Substrate
Im-nNOS
L-Arginine
Product
Im-nNOS-L-Argini
ne
| | Binding of L-Arginine to Imidazole bound rat brain neuronal Nitric Oxide Synthase. Kd = 0.5mM = 500uM. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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