Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.

Search in:

ALL Accession Pathway Molecule Enzyme Reaction Connected to

Search for

Match

Anything Exact Word Starting With

By

Name Notes Model

Molecule Parameter List for Im-nNOS-L-Arginine

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

Im-nNOS-L-Arginine participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
L-Arginine	72	Pathway	L-Arginine
This model was taken from the Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. This model shows kinetic binding of L-Arginine to neuronal nitric oxide synthase. Model shows the substrate (L-Arginine) binding to nNOS in a two-step reversible fashion. First there is rapid binding equilibrium between Im-nNOS and L-Arginine to form an intermediate that contains bound imidazole and L-Arginine. This is followed by a slower conformational change in the Im-enzyme-substrate complex that is associated with release of bound imidazole and generation of a modified enzyme-substrate complex which is detected due to spectral change. Replicates Figure 4 (right panel).

Im-nNOS-L-Arginine acting as a Molecule in  L-Arginine Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Im-nNOS-L-Arginine	L-Arginine Accession No. : 72	L-Arginine Pathway No. : 308	0	0.0016667	No
An intermediate formed due to rapid binding of L-Arginine to Imidazole bound neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51.

Im-nNOS-L-Arginine acting as a Substrate in a reaction in  L-Arginine Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Imidazole_ release	L-Arginine Accession No. : 72	L-Arginine Pathway No. : 308	81 (s^-1)	0.45 (uM^-1 s^-1)	Kd(cb) = 0.0056(uM)	-	Substrate Im-nNOS-L-Argini ne Product Imidazole nNOS-L-Arginine
Release of Imidazole from complex of L-Arginine and Imidazole bound rat brain neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51.

Im-nNOS-L-Arginine acting as a Product in a reaction in  L-Arginine Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Arginine_ binding	L-Arginine Accession No. : 72	L-Arginine Pathway No. : 308	250 (uM^-1 s^-1)	125000 (s^-1)	Kd(bf) = 500(uM)	-	Substrate Im-nNOS L-Arginine Product Im-nNOS-L-Argini ne
Binding of L-Arginine to Imidazole bound rat brain neuronal Nitric Oxide Synthase. Kd = 0.5mM = 500uM. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51.