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Molecule Parameter List for L.EGFR | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2004_Feedback_
Tuning | 78 | Network |
Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning, PKC, PLA2,
PLCbeta, Gq, MAPK,
Ras, EGFR, Sos,
PLC_g, CaMKII, CaM,
PP1, PP2B, PKA,
AC | | This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a. |
L.EGFR acting as a Molecule in Ajay_Bhalla_2004_Feedback_Tuning Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | L.EGFR | Ajay_Bhalla_
2004_Feedback_
Tuning
Accession No. : 78 | EGFR
Pathway No. : 354 | 0 | 1.5 | No | | This is terribly simplified: there are many interesting intermediate stages, including dimerization and assoc with adapter molecules like Shc, that contribute to the activation of the EGFR. |
L.EGFR acting as an Enzyme in Ajay_Bhalla_2004_Feedback_Tuning Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | L.EGFR /
phosph_PLC_g
| Ajay_Bhalla_
2004_Feedback_
Tuning
Accession No. : 78 | EGFR
Pathway No. : 354 | 0.333337 | 0.2 | 4 | explicit E-S complex | Substrate
Ca.PLC_g
Product
Ca.PLC_g*
| | | Hsu et al JBC 266:1 603-608 1991 Km = 385 +- 100 uM, Vm = 5.1 +-1 pmol/min/ug for PLC-771. Other sites have similar range, but are not stim as much by EGF. k1 = 2.8e-2/385/6e5 = 1.2e-10. Phenomenally slow. But Sherrill and Kyte say turnover # for angiotensin II is 5/min for cell extt, and 2/min for placental. Also see Okada et al for Shc rates which are much faster. | | 2 | L.EGFR /
phosph_Shc
| Ajay_Bhalla_
2004_Feedback_
Tuning
Accession No. : 78 | EGFR
Pathway No. : 354 | 0.833354 | 0.2 | 4 | explicit E-S complex | Substrate
SHC
Product
SHC*
| | | Rates from Okada et al JBC 270:35 pp 20737 1995 Km = 0.70 to 0.85 uM, Vmax = 4.4 to 5.0 pmol/min. Unfortunately the amount of enzyme is not known, the prep is only partially purified. Time course of phosph is max within 30 sec, falls back within 20 min. Ref: Sasaoka et al JBC 269:51 32621 1994. Use k3 = 0.1 based on this tau. |
L.EGFR acting as a Substrate in a reaction in Ajay_Bhalla_2004_Feedback_Tuning Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | Internalize | Ajay_Bhalla_
2004_Feedback_
Tuning
Accession No. : 78 | EGFR
Pathway No. : 354 | 0.002
(s^-1) | 0.0003
(s^-1) | Keq = 0.165(uM) | 434.783sec | Substrate
L.EGFR
Product
Internal_L.EGFR
| | See Helin and Beguinot JBC 266:13 1991 pg 8363-8368. In Fig 3 they have internalization tau about 10 min, equil at about 20% EGF available. So kf = 4x kb, and 1/(kf + kb) = 600 sec so kb = 1/3K = 3.3e-4, and kf = 1.33e-3. This doesn't take into account the unbound receptor, so we need to push the kf up a bit, to 0.002 |
L.EGFR acting as a Product in a reaction in Ajay_Bhalla_2004_Feedback_Tuning Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | act_EGFR | Ajay_Bhalla_
2004_Feedback_
Tuning
Accession No. : 78 | EGFR
Pathway No. : 354 | 4.2
(uM^-1 s^-1) | 0.25
(s^-1) | Kd(bf) = 0.0595(uM) | - | Substrate
EGF
EGFR
Product
L.EGFR
| | Affinity of EGFR for EGF is complex: depends on [EGFR]. We'll assume fixed [EGFR] and use exptal affinity ~20 nM (see Sherrill and Kyte Biochem 1996 35 5705-5718, Berkers et al JBC 266:2 922-927 1991, Sorokin et al JBC 269:13 9752-9759 1994). Tau =~2 min (Davis et al JBC 263:11 5373-5379 1988) or Berkers Kass = 6.2e5/M/sec, Kdiss=3.5e-4/sec. Sherrill and Kyte have Hill Coeff=1.7 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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