|
Enter a Search String | Special character and space not allowed in the query term.
Search string should be at least 2 characters long. |
Molecule Parameter List for PKC-cytosolic | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color. | Statistics |
PKC-cytosolic participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction | No. of occurrences | 1 | 0 | 0 | 0 | 0 | 4 | 0 |
Accession and Pathway Details | |
Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_ 2007_Bistable | 79 | Network | Shared_Object_Ajay_Bhalla_2007_Bistable, PKC, PLA2, MAPK, Ras, CaM | This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII. The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80) |
PKC-cytosolic acting as a Molecule in Ajay_Bhalla_2007_Bistable Network
Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | PKC-cytosolic | Ajay_Bhalla_ 2007_Bistable Accession No. : 79 | PKC Pathway No. : 364 | 1 | 125.7 | No | Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells We will use 1 uM as our initial concen |
PKC-cytosolic acting as a Substrate in a reaction in Ajay_Bhalla_2007_Bistable Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | 1 | PKC-act-by-Ca | Ajay_Bhalla_ 2007_Bistable Accession No. : 79 | PKC Pathway No. : 364 | 0.6 (uM^-1 s^-1) | 0.5 (s^-1) | Kd(bf) = 0.8333(uM) | - | Substrate Ca PKC-cytosolic
Product PKC-Ca
| | Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5 | 2 | PKC-basal-act | Ajay_Bhalla_ 2007_Bistable Accession No. : 79 | PKC Pathway No. : 364 | 1 (s^-1) | 50 (s^-1) | Keq = 50(uM) | 0.02sec | Substrate PKC-cytosolic
Product PKC-basal*
| | Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher. | 3 | PKC-act-by-AA | Ajay_Bhalla_ 2007_Bistable Accession No. : 79 | PKC Pathway No. : 364 | 0.0001 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 833.3264(uM) | - | Substrate AA PKC-cytosolic
Product PKC-AA*
| | Raise kf from 1.667e-10 to 2e-10 to get better match to data. | 4 | PKC-n-DAG | Ajay_Bhalla_ 2007_Bistable Accession No. : 79 | PKC Pathway No. : 364 | 0.0006 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 166.6656(uM) | - | Substrate DAG PKC-cytosolic
Product PKC-DAG
| | kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
|