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Molecule Parameter List for PLA2-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLA2-cytosolic participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	1	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_Bistable	79	Network	Shared_Object_Ajay_Bhalla_2007_Bistable,  PKC,  PLA2,   MAPK,  Ras,  CaM
This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII. The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80)

PLA2-cytosolic acting as a Molecule in  Ajay_Bhalla_2007_Bistable Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLA2-cytosolic	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	0.4	125.7	No
Calculated cytosolic was 20 nm from Wijkander and Sundler However, Leslie and Channon use about 400 nM. Need to confirm, but this is the value I use here. Another recalc of W&S gives 1uM

PLA2-cytosolic acting as a Substrate for an Enzyme in  Ajay_Bhalla_2007_Bistable Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MAPK*  / MAPK*	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	25.6402	10	4	explicit E-S complex	Substrate PLA2-cytosolic Product PLA2*
Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

PLA2-cytosolic acting as a Substrate in a reaction in  Ajay_Bhalla_2007_Bistable Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PLA2-Ca-act	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate Ca PLA2-cytosolic Product PLA2-Ca*
	Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.
2	PIP2-PLA2-act	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	0.0012 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 416.6632(uM)	-	Substrate PLA2-cytosolic temp-PIP2 Product PIP2-PLA2*

PLA2-cytosolic acting as a Product in a reaction in  Ajay_Bhalla_2007_Bistable Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
dephosphorylate- PLA2*	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	PLA2 Pathway No. : 365	0.17 (s^-1)	0 (s^-1)	-	-	Substrate PLA2* Product PLA2-cytosolic