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Molecule Parameter List for MAPKK-ser

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MAPKK-ser* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	0	75	75	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_bhalla_ 2007_ReacDiff1_ 1e-12	81	Network	Shared_Object_Ajay_bhalla_2007_ReacDiff1_1e-12, PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1], PKC, MAPK, Ras, CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, kinetics[3], PKC, MAPK, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM, kinetics[5], PKC, MAPK, Ras, kinetics[6], CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[7], PKC, Ras, CaM, PKM, kinetics[8], PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10], PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12], PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14], PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16], PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18], PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20], PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22], PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec. The stimulus file pkm_mapk22_diff_1e-12_Fig4A which was used for the model to replicate Figure 4A from the paper. This stimulus file pkm_mapk22_diff_1e-12_Fig4G which was used for the model to replicate Figure 4G from the paper

MAPKK-ser acting as a Molecule in Ajay_bhalla_2007_ReacDiff1_1e-12 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0	1.5	No
Intermediately phophorylated, assumed inactive, form of MAPKK

MAPKK-ser acting as a Substrate for an Enzyme in Ajay_bhalla_2007_ReacDiff1_1e-12 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
3	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	Shared_Object_ Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Pathway No. : 375	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

4	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
5	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
6	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics Pathway No. : 382	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

7	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
8	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
9	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[1] Pathway No. : 388	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

10	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
11	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
12	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[2] Pathway No. : 394	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

13	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
14	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
15	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[3] Pathway No. : 400	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

16	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
17	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
18	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[4] Pathway No. : 407	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

19	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
20	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
21	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[5] Pathway No. : 413	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

22	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
23	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
24	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[6] Pathway No. : 417	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

25	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
26	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
27	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[7] Pathway No. : 425	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

28	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
29	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
30	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[8] Pathway No. : 430	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

31	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
32	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
33	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[9] Pathway No. : 436	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

34	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
35	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
36	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[10] Pathway No. : 442	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

37	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
38	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
39	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[11] Pathway No. : 448	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

40	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
41	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
42	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[12] Pathway No. : 454	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

43	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
44	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
45	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[13] Pathway No. : 460	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

46	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
47	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
48	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[14] Pathway No. : 466	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

49	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
50	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
51	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[15] Pathway No. : 472	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

52	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
53	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
54	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[16] Pathway No. : 478	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

55	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
56	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
57	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[17] Pathway No. : 484	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

58	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
59	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
60	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[18] Pathway No. : 490	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

61	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
62	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
63	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[19] Pathway No. : 496	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

64	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
65	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
66	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[20] Pathway No. : 502	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

67	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
68	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
69	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[21] Pathway No. : 508	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

70	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
71	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
72	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[22] Pathway No. : 514	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

73	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
74	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
75	PPhosphatase2A / MAPKK-deph-ser	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[23] Pathway No. : 520	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

MAPKK-ser acting as a Product of an Enzyme in Ajay_bhalla_2007_ReacDiff1_1e-12 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
3	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	Shared_Object_ Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Pathway No. : 375	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
4	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
5	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
6	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics Pathway No. : 382	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
7	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
8	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
9	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[1] Pathway No. : 388	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
10	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
11	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
12	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[2] Pathway No. : 394	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
13	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
14	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
15	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[3] Pathway No. : 400	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
16	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
17	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
18	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[4] Pathway No. : 407	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
19	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
20	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
21	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[5] Pathway No. : 413	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
22	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
23	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
24	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[6] Pathway No. : 417	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
25	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
26	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
27	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[7] Pathway No. : 425	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
28	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
29	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
30	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[8] Pathway No. : 430	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
31	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
32	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
33	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[9] Pathway No. : 436	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
34	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
35	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
36	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[10] Pathway No. : 442	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
37	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
38	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
39	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[11] Pathway No. : 448	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
40	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
41	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
42	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[12] Pathway No. : 454	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
43	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
44	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
45	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[13] Pathway No. : 460	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
46	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
47	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
48	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[14] Pathway No. : 466	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
49	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
50	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
51	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[15] Pathway No. : 472	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
52	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
53	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
54	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[16] Pathway No. : 478	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
55	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
56	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
57	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[17] Pathway No. : 484	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
58	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
59	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
60	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[18] Pathway No. : 490	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
61	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
62	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
63	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[19] Pathway No. : 496	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
64	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
65	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
66	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[20] Pathway No. : 502	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
67	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
68	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
69	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[21] Pathway No. : 508	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
70	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
71	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
72	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[22] Pathway No. : 514	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
73	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
74	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
75	PPhosphatase2A / MAPKK-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[23] Pathway No. : 520	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

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