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Molecule Parameter List for GTP-Ras | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_bhalla_
2007_ReacDiff1_
1e-12 | 81 | Network | Shared_Object_Ajay_bhalla_2007_ReacDiff1_1e-12,
PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1],
PKC, MAPK, Ras, CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, kinetics[3],
PKC, MAPK, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM,
kinetics[5], PKC, MAPK, Ras, kinetics[6], CaM, PKM, PKC, MAPK, Ras,
CaM, PKM, kinetics[7], PKC, Ras, CaM, PKM, kinetics[8], PKC, MAPK,
Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10],
PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12],
PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14],
PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16],
PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18],
PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20],
PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22],
PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM | This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec.
The stimulus file pkm_mapk22_diff_1e-12_Fig4A which was used for the model to replicate Figure 4A from the paper.
This stimulus file pkm_mapk22_diff_1e-12_Fig4G which was used for the model to replicate Figure 4G from the paper |
GTP-Ras acting as a Molecule in Ajay_bhalla_2007_ReacDiff1_1e-12 Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 378 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 385 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 391 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 397 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 404 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 410 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 416 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 422 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 427 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 433 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 439 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 445 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 451 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 457 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 463 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 469 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 475 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 481 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 487 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 493 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 499 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 505 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 511 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 517 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 | | GTP-Ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 523 | 0 | 1.5 | No | | Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437 |
GTP-Ras acting as a Substrate for an Enzyme in Ajay_bhalla_2007_ReacDiff1_1e-12 Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 378 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 2 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 385 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 3 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 391 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 4 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 397 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 5 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 404 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 6 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 410 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 7 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 416 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 8 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 422 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 9 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 427 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 10 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 433 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 11 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 439 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 12 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 445 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 13 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 451 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 14 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 457 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 15 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 463 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 16 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 469 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 17 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 475 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 18 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 481 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 19 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 487 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 20 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 493 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 21 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 499 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 22 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 505 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 23 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 511 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 24 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 517 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | | 25 | GAP /
GAP-inact-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 523 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate
GTP-Ras
Product
GDP-Ras
| | | From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. |
GTP-Ras acting as a Product of an Enzyme in Ajay_bhalla_2007_ReacDiff1_1e-12 Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 378 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 2 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 378 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 3 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 378 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 4 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 385 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 5 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 385 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 6 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 385 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 7 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 391 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 8 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 391 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 9 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 391 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 10 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 397 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 11 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 397 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 12 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 397 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 13 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 404 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 14 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 404 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 15 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 404 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 16 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 410 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 17 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 410 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 18 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 410 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 19 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 416 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 20 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 416 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 21 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 416 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 22 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 422 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 23 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 422 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 24 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 422 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 25 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 427 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 26 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 427 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 27 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 427 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 28 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 433 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 29 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 433 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 30 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 433 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 31 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 439 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 32 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 439 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 33 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 439 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 34 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 445 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 35 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 445 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 36 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 445 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 37 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 451 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 38 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 451 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 39 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 451 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 40 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 457 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 41 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 457 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 42 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 457 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 43 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 463 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 44 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 463 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 45 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 463 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 46 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 469 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 47 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 469 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 48 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 469 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 49 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 475 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 50 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 475 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 51 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 475 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 52 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 481 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 53 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 481 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 54 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 481 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 55 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 487 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 56 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 487 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 57 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 487 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 58 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 493 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 59 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 493 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 60 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 493 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 61 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 499 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 62 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 499 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 63 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 499 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 64 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 505 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 65 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 505 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 66 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 505 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 67 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 511 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 68 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 511 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 69 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 511 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 70 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 517 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 71 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 517 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 72 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 517 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | | 73 | inact-GEF /
basal_GEF_
activity
| Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 523 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | | | 74 | GEF* /
GEF*-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 523 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg-act-ras | | 75 | CaM-GEF /
CaM-GEF-act-ras | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 523 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate
GDP-Ras
Product
GTP-Ras
| | | Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. |
GTP-Ras acting as a Substrate in a reaction in Ajay_bhalla_2007_ReacDiff1_1e-12 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Shared_Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12
Pathway No. : 375 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 2 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 378 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 3 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Shared_Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12
Pathway No. : 375 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 4 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics
Pathway No. : 382 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 5 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 385 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 6 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics
Pathway No. : 382 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 7 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[1]
Pathway No. : 388 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 8 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 391 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 9 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[1]
Pathway No. : 388 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 10 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[2]
Pathway No. : 394 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 11 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 397 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 12 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[2]
Pathway No. : 394 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 13 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[3]
Pathway No. : 400 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 14 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 404 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 15 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[3]
Pathway No. : 400 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 16 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[4]
Pathway No. : 407 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 17 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 410 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 18 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[4]
Pathway No. : 407 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 19 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[5]
Pathway No. : 413 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 20 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 416 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 21 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[5]
Pathway No. : 413 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 22 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[6]
Pathway No. : 417 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 23 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 422 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 24 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[6]
Pathway No. : 417 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 25 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[7]
Pathway No. : 425 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 26 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 427 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 27 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[7]
Pathway No. : 425 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 28 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[8]
Pathway No. : 430 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 29 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 433 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 30 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[8]
Pathway No. : 430 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 31 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[9]
Pathway No. : 436 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 32 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 439 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 33 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[9]
Pathway No. : 436 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 34 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[10]
Pathway No. : 442 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 35 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 445 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 36 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[10]
Pathway No. : 442 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 37 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[11]
Pathway No. : 448 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 38 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 451 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 39 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[11]
Pathway No. : 448 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 40 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[12]
Pathway No. : 454 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 41 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 457 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 42 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[12]
Pathway No. : 454 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 43 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[13]
Pathway No. : 460 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 44 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 463 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 45 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[13]
Pathway No. : 460 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 46 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[14]
Pathway No. : 466 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 47 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 469 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 48 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[14]
Pathway No. : 466 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 49 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[15]
Pathway No. : 472 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 50 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 475 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 51 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[15]
Pathway No. : 472 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 52 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[16]
Pathway No. : 478 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 53 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 481 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 54 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[16]
Pathway No. : 478 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 55 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[17]
Pathway No. : 484 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 56 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 487 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 57 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[17]
Pathway No. : 484 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 58 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[18]
Pathway No. : 490 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 59 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 493 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 60 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[18]
Pathway No. : 490 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 61 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[19]
Pathway No. : 496 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 62 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 499 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 63 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[19]
Pathway No. : 496 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 64 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[20]
Pathway No. : 502 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 65 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 505 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 66 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[20]
Pathway No. : 502 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 67 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[21]
Pathway No. : 508 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 68 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 511 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 69 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[21]
Pathway No. : 508 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 70 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[22]
Pathway No. : 514 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 71 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 517 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 72 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[22]
Pathway No. : 514 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. | | 73 | Ras-act-craf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[23]
Pathway No. : 520 | 9.9998
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.05(uM) | - | Substrate
GTP-Ras
craf-1*
Product
Raf*-GTP-Ras
| | | Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10. | | 74 | Ras-intrinsic-GT
Pase | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | Ras
Pathway No. : 523 | 0.0001
(s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
Product
GDP-Ras
| | | This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | | 75 | Ras-act-unphosph
-raf | Ajay_bhalla_
2007_ReacDiff1_
1e-12
Accession No. : 81 | kinetics[23]
Pathway No. : 520 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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