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Molecule Parameter List for PKC-active

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PKC-active* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	25	75	0	0	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff3	84	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff3, PKC, PLA2, MAPK, PLA2, Ras, CaM, chain, kinetics, PKC, MAPK, Ras, CaM, kinetics[1], PKC, PLA2, MAPK, Ras, CaM, kinetics[2], PKC, PLA2, MAPK, Ras, CaM, kinetics[3], PKC, PLA2, MAPK, Ras, CaM, kinetics[4], PKC, PLA2, MAPK, Ras, CaM, kinetics[5], PKC, PLA2, MAPK, Ras, MAPK, CaM, kinetics[6], PKC, PLA2, MAPK, Ras, CaM, kinetics[7], PKC, PLA2, MAPK, Ras, CaM, PKC, kinetics[8], PLA2, MAPK, Ras, CaM, kinetics[9], PKC, PLA2, MAPK, Ras, CaM, kinetics[10], PKC, PLA2, MAPK, Ras, CaM, kinetics[11], PKC, PLA2, MAPK, Ras, CaM, kinetics[12], PKC, PLA2, Ras, CaM, kinetics[13], PKC, PLA2, MAPK, Ras, CaM, kinetics[14], PKC, PLA2, MAPK, Ras, CaM, kinetics[15], PKC, PLA2, MAPK, Ras, kinetics[16], CaM, PKC, PLA2, MAPK, Ras, CaM, kinetics[17], PKC, PLA2, MAPK, Ras, CaM, kinetics[18], PKC, PLA2, MAPK, Ras, CaM, kinetics[19], PKC, PLA2, MAPK, Ras, CaM, kinetics[20], PKC, PLA2, MAPK, Ras, CaM, kinetics[21], PKC, PLA2, MAPK, Ras, CaM, kinetics[22], PKC, PLA2, MAPK, Ras, CaM, kinetics[23], PKC, PLA2, MAPK, Ras, CaM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times. In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec. The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation. The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

PKC-active acting as a Molecule in Ajay_Bhalla_2007_ReacDiff3 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	0.3	125.7	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	0.3	125.7	No

PKC-active acting as a Summed Molecule in Ajay_Bhalla_2007_ReacDiff3 Network

	Accession Nane	Pathway Name	Target	Input
1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	PKC-active	PKC-DAG-AA* PKC-Ca-memb* PKC-Ca-AA* PKC-DAG-memb* PKC-basal* PKC-AA*

2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

3	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

4	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

5	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

6	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

7	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

8	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

9	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

10	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

11	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

12	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

13	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

14	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

15	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

16	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

17	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

18	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

19	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

20	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

21	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

22	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

23	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

24	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

25	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

PKC-active acting as an Enzyme in Ajay_Bhalla_2007_ReacDiff3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
2	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
3	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
4	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
5	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
6	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
7	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
8	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
9	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
10	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
11	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
12	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
13	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
14	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
15	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
16	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
17	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
18	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
19	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
20	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
21	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
22	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
23	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
24	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
25	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
26	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
27	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
28	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
29	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
30	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
31	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
32	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
33	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
34	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
35	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
36	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
37	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
38	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
39	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
40	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
41	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
42	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
43	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
44	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
45	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
46	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
47	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
48	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
49	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
50	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
51	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
52	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
53	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
54	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
55	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
56	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
57	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
58	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
59	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
60	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
61	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
62	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
63	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
64	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
65	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
66	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
67	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
68	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
69	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
70	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
71	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
72	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
73	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	66.6665	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
74	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
75	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X

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