| |
Reaction
Name | Pathway Name /
Pathway No. | Kf | Kb | Kd | tau | Reagents |
| 1 | DAG-Ca-PLA2-act | PLA2
Pathway No. 920 | 0.003
(uM^-1 s^-1) | 4
(s^-1) | Kd(bf) = 1333.3289(uM) | - | Substrate:
DAG
PLA2-Ca*
Products:
DAG-Ca-PLA2*
|
| 27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2. | | 2 | PIP2-PLA2-act | PLA2
Pathway No. 920 | 0.0012
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 416.6667(uM) | - | Substrate:
temp-PIP2
PLA2-cytosolic
Products:
PIP2-PLA2*
|
| 3 | PLA2-Ca-act | PLA2
Pathway No. 920 | 1
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.1(uM) | - | Substrate:
PLA2-cytosolic
Ca
Products:
PLA2-Ca*
|
| Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267. | | 4 | PIP2-Ca-PLA2-act | PLA2
Pathway No. 920 | 0.012
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 8.3333(uM) | - | Substrate:
temp-PIP2
PLA2-Ca*
Products:
PIP2-Ca-PLA2*
|
| 5 | PLA2*-Ca-act | PLA2
Pathway No. 920 | 6
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.0167(uM) | - | Substrate:
PLA2*
Ca
Products:
PLA2*-Ca
|
| To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien.... | | 6 | Degrade-AA | PLA2
Pathway No. 920 | 0.4
(s^-1) | 0
(s^-1) | - | - | Substrate:
AA
Products:
APC
|
| I need to check if the AA degradation pathway really leads back to APC. Anyway, it is a convenient buffered pool to dump it back into. For the purposes of the full model we use a rate of degradation of 0.2/sec Raised decay to 0.4 : see PLA35.g notes for Feb17 | | 7 | dephosphorylate-
PLA2* | PLA2
Pathway No. 920 | 0.17
(s^-1) | 0
(s^-1) | - | - | Substrate:
PLA2*
Products:
PLA2-cytosolic
|
and 
color.
