| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | 5prime_GMP | 0 | 0.0016667 | No |
| | |
| 2 | cGMP | 0 | 0.0016667 | No |
| | |
| 3 | cGMP.PKG | 0 | 0.0016667 | No |
| | |
| 4 | GC | 3 | 0.0016667 | Yes |
| | Guanylyl Cyclase ... Intracellular concentration is around 3 uM, as sGC is enrcihed in the Purkinje cells (Shinya Kuroda, personal corresspondence).. Conc assumed on the basis of reported data in Ariano et al., 1982, PNAS,79:297-300. |
| 5 | Gsub | 10.7 | 0.0016667 | No |
| | Conc from Detre et al., 1984, J Neurosci, 4:2843-2849. |
| 6 | Gsub* | 0 | 0.0016667 | No |
| | |
| 7 | GTP | 10 | 0.0016667 | No |
| | conc used from Bhalla and Iyengar,1999,Science, 283:381-387.. |
| 8 | NO | 0.1 | 0.0016667 | No |
| | endogenous NO concentration is the course of signal transduction processes are around ~100 nM .. (Varner et al., Nitric Oxide in the Nervous System, Academic Press, pp:191-206) |
| 9 | NOdegrade | 0 | 0.0016667 | No |
| | |
| 10 | NO_GC | 0 | 0.0016667 | No |
| | |
| 11 | PDE | 5 | 0.0016667 | No |
| | PhophoDiesterase ... Degrades cGMP to 5'GMP ... Conc Initially used from Kotera et al.,1997, Eur J Biochem, 249:434-442. |
| 12 | PKG | 2.5 | 0.0016667 | No |
| | PKG inactive ... conc ~2.5 uM ..Lohmann et al., PNAS, 1981, 78:635-657. and Shinya Kuroda, personal correspondence .... |
| 13 | PP2A | 2.7 | 0.0016667 | No |
| | conc from Mumby et al., 1985, J Biol Chem, 260:13763-13770. |
| 14 | PP2A_Gsub* | 0 | 0.0016667 | No |
| | |
