DOQCS database

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.

	Name	Kf	Kb	Kd	tau	Substrate	Product
1	DAG-Ca-PLA2-act	0.003 (uM^-1 s^-1)	4 (s^-1)	Kd(bf) = 1333.3289(uM)	-	PLA2-Ca* DAG	DAG-Ca-PLA2*
	27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
2	Degrade-AA	0.4 (s^-1)	0 (s^-1)	-	-	AA	APC
	I need to check if the AA degradation pathway really leads back to APC. Anyway, it is a convenient buffered pool to dump it back into. For the purposes of the full model we use a rate of degradation of 0.2/sec Raised decay to 0.4 : see PLA35.g notes for Feb17
3	dephosphorylate- PLA2*	0.17 (s^-1)	0 (s^-1)	-	-	PLA2*	PLA2-cytosolic
4	PIP2-Ca-PLA2-act	0.012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 8.3333(uM)	-	PLA2-Ca* temp-PIP2	PIP2-Ca-PLA2*
5	PIP2-PLA2-act	0.0012 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 416.6667(uM)	-	PLA2-cytosolic temp-PIP2	PIP2-PLA2*
6	PLA2*-Ca-act	6 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.0167(uM)	-	PLA2* Ca	PLA2*-Ca
	To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
7	PLA2-Ca-act	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	PLA2-cytosolic Ca	PLA2-Ca*
	Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.

Pathway Details Molecule List Enzyme List Reaction List

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