Enter a Search String |
| Special character and space not allowed in the query term. Search string should be at least 2 characters long. |
Reaction List for pathway 134_dephos (Pathway Number 165) |
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| Name | Kf | Kb | Kd | tau | Substrate | Product | |
| 1 | 1pase-off | 11.43 (s^-1) | 0.0008 (uM^-1 s^-1) | Kd(cb) = 0.0001(uM) | - | ip1_1pase_cmplx | inositol IP1_pase |
| Kf = Vmax for Ins(1)P1-1phosphatase: Gee et al, Biochem J 249, 1988. Kb necessary as estimated from percent inositol backflux calculations. This contributes to maintain Ins(1)P1 at 10% of its actual pool. | |||||||
| 2 | 1pase-on | 0.3572 (uM^-1 s^-1) | 45.72 (s^-1) | Kd(bf) = 128.0005(uM) | - | IP1(1) IP1_pase | ip1_1pase_cmplx |
| Rates derived from Km for Ins(1)P1-1phosphatase: Gee et al, Biochem J 249; 1988. | |||||||
| 3 | IP1(3)_deg | 35 (s^-1) | 0.336 (s^-1) | Keq = 0.0096(uM) | 0.028sec | IP1(3) | inositol |
| Kf and Kb based on levels of Ins(3)P1. Kb necessary as energetics calculations show backflow from inositol to be significant. | |||||||
| 4 | ip1_syn | 0.11 (s^-1) | 0 (s^-1) | - | - | inositol | IP1(1) |
| This rxn. maintains levels of Ins(1)P1 at 44 uM basal. Actually, 90% of Ins(1)P1 is generated from PtdIns degradation (Ackermann et al, Biochem J 242(2), 1987: 517-24) but for simplicity we use a back rxn. from Inositol to generate this conc. | |||||||
| 5 | IP_4pase-inact | 1 (uM^-1 s^-1) | 19 (s^-1) | Kd(bf) = 19(uM) | - | IP_4pase IP6 | IP_4pase_inact |
| from Norris et al, JBC 269; 1994 | |||||||
| Pathway Detail | Molecule List | Enzyme List | Reaction List |