DOQCS database

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.

	Name	Kf	Kb	Kd	tau	Substrate	Product
1	PKC-act-by-Ca	0.6 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.8333(uM)	-	PKC-cytosolic Ca	PKC-Ca
	Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
2	PKC-act-by-DAG	0.008 (uM^-1 s^-1)	8.6348 (s^-1)	Kd(bf) = 1079.4148(uM)	-	PKC-Ca DAG	PKC-Ca-DAG
	Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
3	PKC-Ca-to-memb	1.2705 (s^-1)	3.5026 (s^-1)	Keq = 2.7569(uM)	0.21sec	PKC-Ca	PKC-Ca-memb*
4	PKC-DAG-to-memb	1 (s^-1)	0.1 (s^-1)	Keq = 0.1(uM)	0.909sec	PKC-Ca-DAG	PKC-DAG-memb*
	Raise kb from .087 to 0.1 to match data from Shinomura et al. Lower kf from 1.155 to 1.0 to match data from Shinomura et al.
5	PKC-n-DAG	0.0006 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 166.6664(uM)	-	PKC-cytosolic DAG	PKC-DAG
	kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9

Pathway Details Molecule List Enzyme List Reaction List

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