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Reaction Name | Pathway Name / Pathway No.![](database/fig/up.png) | Kf | Kb | Kd | tau | Reagents |
1 | PLA2-Ca-act | PLA2
Pathway No. 1053 | 1 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.1(uM) | - | Substrate: PLA2-cytosolic Ca
Products: PLA2-Ca*
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| Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267. | 2 | PIP2-PLA2-act | PLA2
Pathway No. 1053 | 0.0012 (uM^-1 s^-1) | 0.5 (s^-1) | Kd(bf) = 416.6667(uM) | - | Substrate: PLA2-cytosolic temp-PIP2
Products: PIP2-PLA2*
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3 | PIP2-Ca-PLA2-act | PLA2
Pathway No. 1053 | 0.012 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 8.3333(uM) | - | Substrate: PLA2-Ca* temp-PIP2
Products: PIP2-Ca-PLA2*
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4 | DAG-Ca-PLA2-act | PLA2
Pathway No. 1053 | 0.003 (uM^-1 s^-1) | 4 (s^-1) | Kd(bf) = 1333.3289(uM) | - | Substrate: PLA2-Ca* DAG
Products: DAG-Ca-PLA2*
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| 27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2. | 5 | Degrade-AA | PLA2
Pathway No. 1053 | 0.4 (s^-1) | 0 (s^-1) | - | - | Substrate: AA
Products: APC
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| I need to check if the AA degradation pathway really leads back to APC. Anyway, it is a convenient buffered pool to dump it back into. For the purposes of the full model we use a rate of degradation of 0.2/sec Raised decay to 0.4 : see PLA35.g notes for Feb17 | 6 | PLA2*-Ca-act | PLA2
Pathway No. 1053 | 6 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0167(uM) | - | Substrate: PLA2* Ca
Products: PLA2*-Ca
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| To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien.... | 7 | dephosphorylate- PLA2* | PLA2
Pathway No. 1053 | 0.17 (s^-1) | 0 (s^-1) | - | - | Substrate: PLA2*
Products: PLA2-cytosolic
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