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Reaction Name | Pathway Name / Pathway No.![](database/fig/up.png) | Kf | Kb | Kd | tau | Reagents |
1 | PKC-act-by-Ca | PKC
Pathway No. 938 | 0.6 (uM^-1 s^-1) | 0.5 (s^-1) | Kd(bf) = 0.8333(uM) | - | Substrate: PKC-cytosolic Ca
Products: PKC-Ca
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| Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5 | 2 | PKC-act-by-DAG | PKC
Pathway No. 938 | 0.008 (uM^-1 s^-1) | 8.6348 (s^-1) | Kd(bf) = 1079.4148(uM) | - | Substrate: PKC-Ca DAG
Products: PKC-Ca-DAG
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| Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8 | 3 | PKC-Ca-to-memb | PKC
Pathway No. 938 | 1.2705 (s^-1) | 3.5026 (s^-1) | Keq = 2.7569(uM) | 0.21sec | Substrate: PKC-Ca
Products: PKC-Ca-memb*
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4 | PKC-DAG-to-memb | PKC
Pathway No. 938 | 1 (s^-1) | 0.1 (s^-1) | Keq = 0.1(uM) | 0.909sec | Substrate: PKC-Ca-DAG
Products: PKC-DAG-memb*
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| Raise kb from .087 to 0.1 to match data from Shinomura et al. Lower kf from 1.155 to 1.0 to match data from Shinomura et al. | 5 | PKC-act-by-Ca-AA | PKC
Pathway No. 938 | 0.0012 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 83.3333(uM) | - | Substrate: PKC-Ca AA
Products: PKC-Ca-AA*
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| Schaechter and Benowitz We have to increase Kf for conc scaling Changed kf to 2e-9 on Sept 19, 94. This gives better match. | 6 | PKC-act-by-DAG-A A | PKC
Pathway No. 938 | 2 (s^-1) | 0.2 (s^-1) | Keq = 0.1(uM) | 0.455sec | Substrate: PKC-DAG-AA
Products: PKC-DAG-AA*
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| Assume slowish too. Schaechter and Benowitz | 7 | PKC-basal-act | PKC
Pathway No. 938 | 1 (s^-1) | 50 (s^-1) | Keq = 50(uM) | 0.02sec | Substrate: PKC-cytosolic
Products: PKC-basal*
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| Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher. | 8 | PKC-act-by-AA | PKC
Pathway No. 938 | 0.0001 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 833.3333(uM) | - | Substrate: PKC-cytosolic AA
Products: PKC-AA*
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| Raise kf from 1.667e-10 to 2e-10 to get better match to data. | 9 | PKC-n-DAG | PKC
Pathway No. 938 | 0.0006 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 166.6664(uM) | - | Substrate: PKC-cytosolic DAG
Products: PKC-DAG
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| kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9 | 10 | PKC-n-DAG-AA | PKC
Pathway No. 938 | 0.018 (uM^-1 s^-1) | 2 (s^-1) | Kd(bf) = 111.1111(uM) | - | Substrate: PKC-DAG AA
Products: PKC-DAG-AA
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| Reduced kf to 0.66X to match Shinomura et al data. Initial: kf = 3.3333e-9 New: 2e-9 Newer: 2e-8 kb was 0.2 now 2. |