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Molecule Parameter List for CaMKII-thr286*-CaM

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CaMKII-thr286*-CaM participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1101201

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Osc_Ca_
    IP3metabolism
  • 24Network
    MIPP CaMKII CaM 
    PKC IP3-3K Gq 
    PLCbeta 134_dephos 145_dephos 
    IP4-system IHP-system 1345_dephos 
    CaRegulation Othmer-Tang-model 
    This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    CaMKII-thr286*-CaM acting as a Molecule in  
    Osc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    CaMKII-thr286*-CaM
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • CaMKII
    Pathway No. : 121
    01000No
    From Hanson and Schulman, the thr286 is responsible for autonomous activation of CaMKII.

    CaMKII-thr286*-CaM acting as a Summed Molecule in  
    Osc_Ca_IP3metabolism Network
    Accession NamePathway NameTargetInput
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • CaMKII
    Pathway No. : 121
    tot_CaM_CaMKIICaMKII-CaM
  • CaMKII-thr286*-C
    aM

  • This pool sums the levels of the CaM-bound forms of CaMKII: CaMKII-CaM + CaMKII-thr286*-CaM. Although their phosphorylation states are different, the level of activity is about the same so it makes sense to sum the levels. Hanson et al 1994 Neuron 12:943-956

    CaMKII-thr286*-CaM acting as a Substrate for an Enzyme in  
    Osc_Ca_IP3metabolism Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PP1-active  /
    Deph-thr286
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • CaMKII
    Pathway No. : 121
    5.099070.354explicit E-S complexSubstrate
  • CaMKII-thr286*-C
    aM


    Product
    CaMKII-CaM
  • The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35. This gives the final Km of 5.1, and Vmax of 0.35/sec.

    CaMKII-thr286*-CaM acting as a Product of an Enzyme in  
    Osc_Ca_IP3metabolism Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1tot_CaM_CaMKII  /
    CaM_act_286
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • CaMKII
    Pathway No. : 121
    0.000002705630.54explicit E-S complexSubstrate
    CaMKII-CaM

    Product
  • CaMKII-thr286*-C
    aM

  •     See Hanson and Schulman 1992 JBC 267(24):17216-17224
    2
  • tot_autonomous_
    CaMKII
      /
    auton_286
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • CaMKII
    Pathway No. : 121
    0.000004166670.54explicit E-S complexSubstrate
    CaMKII-CaM

    Product
  • CaMKII-thr286*-C
    aM

  •     The autonomous rate has a slightly higher Km than the CaM-bound rate, but Vmax is the same. Hanson and Schulman 1992 Ann Rev Biochem 61:559-601 and Hanson and Schulman 1992 JBC 267(24):17216-17224

    CaMKII-thr286*-CaM acting as a Product in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • CaMK-thr286-bind
    -CaM
  • Osc_Ca_
    IP3metabolism

    Accession No. : 24
  • CaMKII
    Pathway No. : 121
    1000.2
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0001(uM)-Substrate
    CaM-Ca4
    CaMKII-thr286

    Product
  • CaMKII-thr286*-C
    aM

  • Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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