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Molecule List for pathway PLA2 (Pathway Number 72) in Accession Synaptic_Network (Accession Number 16)

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The entries are grouped according to Pathway Number and are alternately color coded using  and  color.

BufferedSum Total Of
1 APCNetwork300Yes
    arachodonylphosphatidylcholine is the favoured substrate from Wijkander and Sundler, JBC 202 pp 873-880, 1991. Their assay used 30 uM substrate, which is what the kinetics in this model are based on. For the later model we should locate a more realistic value for APC. For now it is treated as a buffered metabolite.
2 DAG-Ca-PLA2*Network00No
    Active form of PLA2 with DAG and Ca bound. DAG and Ca act in combination hence the need for this form in the model. Leslie and Channon (199) BBA 1045:261-270
3 PIP2-Ca-PLA2*Network00No
    Activated form of PLA2 with PIP2 and Ca bound.
4 PIP2-PLA2*Network00No
    PLA2 activated by PIP2 alone.
5 PLA2*Network00No
    Phosphorylated PLA2. The site differs from the site phosphorylated by PKC. See Nemenoff et al 1993 JBC 268(3):1960-1964
6 PLA2*-CaNetwork00No
    Phosphorylated and active form of PLA2. Several kinases act on it: PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone The Nemenoff assays are conducted in rather high Ca so I have assumed a Ca binding step.
7 PLA2-Ca*Network00No
    The generic Ca-activated form ofPLA2. Leslie and Channon 1990 BBA 1045:261.
8 PLA2-cytosolicNetwork0.40No
    cPLA2 IV form has mol wt of 85 Kd. Glaser et al 1993 TIPS 14:92-98. Calculated cytosolic concentration is ~300 nM from Wijkander and Sundler 1991 Eur J Biochem 202:873 Leslie and Channon 1990 BBA 1045:261 use about 400 nM. Decent match. Use 400 nM.

Result: 1 - 8 of 8 rows are displayed

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