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Molecule Parameter List for CaMKII*** | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Osc_Ca_
IP3metabolism | 24 | Network |
MIPP, CaMKII, CaM,
PKC, IP3-3K, Gq,
PLCbeta, 134_dephos, 145_dephos,
IP4-system, IHP-system, 1345_dephos,
CaRegulation, Othmer-Tang-model | | This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. |
CaMKII*** acting as a Molecule in Osc_Ca_IP3metabolism Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | CaMKII*** | Osc_Ca_
IP3metabolism
Accession No. : 24 | CaMKII
Pathway No. : 121 | 0 | 1000 | No | | From Hanson and Schulman, the CaMKII does a lot of autophosphorylation just after the CaM is released. This prevents further CaM binding and renders the enzyme quite independent of Ca. |
CaMKII*** acting as a Summed Molecule in Osc_Ca_IP3metabolism Network
| Accession Name | Pathway Name | Target | Input | Osc_Ca_
IP3metabolism
Accession No. : 24 | CaMKII
Pathway No. : 121 | tot_autonomous_CaMKII | CaMKII-thr286
CaMKII***
| | This is the sum total of the various CaM-independent forms of the kinase. There are actually several possible states here, but I only consider the forms thr-286 phosphorylated form and the doubly/triply phosphorylated form including the thr305/306, represented here as CaMKII*** |
CaMKII*** acting as a Substrate for an Enzyme in Osc_Ca_IP3metabolism Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | PP1-active /
Deph-thr305 | Osc_Ca_
IP3metabolism
Accession No. : 24 | CaMKII
Pathway No. : 121 | 5.09907 | 0.35 | 4 | explicit E-S complex | Substrate
CaMKII***
Product
CaMKII-thr286
| | | Dephosphorylation tempkin are assumed to be the same for all phosphorylation sites on CaMKII. The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35. This gives the final Km of 5.1, and Vmax of 0.35/sec. | | 2 | PP1-active /
Deph-thr286c | Osc_Ca_
IP3metabolism
Accession No. : 24 | CaMKII
Pathway No. : 121 | 5.09907 | 0.35 | 4 | explicit E-S complex | Substrate
CaMKII***
Product
CaMK-thr306
| | | Dephosphorylation tempkin are assumed to be the same for all phosphorylation sites on CaMKII. The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35. This gives the final Km of 5.1, and Vmax of 0.35/sec. |
CaMKII*** acting as a Product of an Enzyme in Osc_Ca_IP3metabolism Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | tot_CaM_CaMKII /
CaM_act_305 | Osc_Ca_
IP3metabolism
Accession No. : 24 | CaMKII
Pathway No. : 121 | 0.00000270563 | 6 | 4 | explicit E-S complex | Substrate
CaMKII-thr286
Product
CaMKII***
| | | Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. See especially Fig 5. | | 2 | tot_autonomous_
CaMKII /
auton_305 | Osc_Ca_
IP3metabolism
Accession No. : 24 | CaMKII
Pathway No. : 121 | 0.00000416667 | 6 | 4 | explicit E-S complex | Substrate
CaMKII-thr286
Product
CaMKII***
| | | See Hanson and Schulman 1992 JBC 267(24):17216-17224 for afterburst rates of phosphorylation |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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