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Molecule Parameter List for MAPKK

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MAPKK participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences2500502500

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff3
    		• 84NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff3,  PKC,  PLA2,  
    MAPK,  PLA2,  Ras,  CaM,  chain,  kinetics,  PKC,  MAPK,  Ras,  CaM,  kinetics[1],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[2],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[3],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[4],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[5],  
    PKC,  PLA2,  MAPK,  Ras,  MAPK,  CaM,  kinetics[6],  PKC,  PLA2,  MAPK,  Ras,  
    CaM,  kinetics[7],  PKC,  PLA2,  MAPK,  Ras,  CaM,  PKC,  kinetics[8],  PLA2,  
    MAPK,  Ras,  CaM,  kinetics[9],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[10],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[11],  PKC,  PLA2,  MAPK,  Ras,  CaM,  
    kinetics[12],  PKC,  PLA2,  Ras,  CaM,  kinetics[13],  PKC,  PLA2,  MAPK,  
    Ras,  CaM,  kinetics[14],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[15],  
    PKC,  PLA2,  MAPK,  Ras,  kinetics[16],  CaM,  PKC,  PLA2,  MAPK,  Ras,  CaM,  
    kinetics[17],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[18],  PKC,  PLA2,  
    MAPK,  Ras,  CaM,  kinetics[19],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[20],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[21],  PKC,  PLA2,  MAPK,  Ras,  CaM,  
    kinetics[22],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[23],  PKC,  PLA2,  
    MAPK,  Ras,  CaM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times.
    In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec.
    The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation.
    The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

    MAPKK acting as a Molecule in      Ajay_Bhalla_2007_ReacDiff3 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 921    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 928    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 934    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 940    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 946    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 952    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 958    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 960    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 965    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 971    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 977    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 983    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 989    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 995    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1006    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1012    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1018    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1024    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1030    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1036    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1042    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1048    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1054    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1060    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
    MAPKK
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1066    		0.5125.7No
    Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.

    MAPKK acting as a Substrate for an Enzyme in      Ajay_Bhalla_2007_ReacDiff3 Network
     Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 921    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    2Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 921    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    3Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 928    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    4Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 928    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    5Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 934    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    6Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 934    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    7Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 940    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    8Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 940    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    9Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 946    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    10Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 946    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    11Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 952    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    12Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 952    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    13Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 958    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    14Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 958    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    15Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 965    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    16Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 965    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    17Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 971    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    18Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 971    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    19Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 977    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    20Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 977    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    21Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 983    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    22Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 983    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    23Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 989    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    24Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 989    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    25Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 995    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    26Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 995    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    27Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 960    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    28Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 960    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    29Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1006    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    30Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1006    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    31Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1012    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    32Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1012    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    33Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1018    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    34Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1018    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    35Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1024    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    36Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1024    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    37Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1030    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    38Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1030    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    39Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1036    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    40Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1036    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    41Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1042    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    42Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1042    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    43Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1048    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    44Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1048    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    45Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1054    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    46Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1054    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    47Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1060    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    48Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1060    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    49Raf-GTP-Ras  /
    Raf-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1066    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    50Raf*-GTP-Ras  /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• MAPK
    Pathway No. : 1066    		0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6

    MAPKK acting as a Product of an Enzyme in      Ajay_Bhalla_2007_ReacDiff3 Network
     Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3
    Pathway No. : 918
    		• 15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    2PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics
    Pathway No. : 926    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    3PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[1]
    Pathway No. : 931    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    4PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[2]
    Pathway No. : 937    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    5PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[3]
    Pathway No. : 943    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    6PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[4]
    Pathway No. : 949    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    7PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[5]
    Pathway No. : 955    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    8PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[6]
    Pathway No. : 962    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    9PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[7]
    Pathway No. : 968    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    10PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[8]
    Pathway No. : 975    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    11PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[9]
    Pathway No. : 980    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    12PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[10]
    Pathway No. : 986    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    13PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[11]
    Pathway No. : 992    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    14PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[12]
    Pathway No. : 998    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    15PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[13]
    Pathway No. : 1003    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    16PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[14]
    Pathway No. : 1009    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    17PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[15]
    Pathway No. : 1015    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    18PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[16]
    Pathway No. : 1020    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    19PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[17]
    Pathway No. : 1027    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    20PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[18]
    Pathway No. : 1033    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    21PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[19]
    Pathway No. : 1039    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    22PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[20]
    Pathway No. : 1045    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    23PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[21]
    Pathway No. : 1051    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    24PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[22]
    Pathway No. : 1057    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       
    25PPhosphatase2A  /
    MAPKK-deph-ser
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[23]
    Pathway No. : 1063    		15.656764explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK
       


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