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Molecule Parameter List for MAPKK*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

MAPKK* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	50	25	50	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff3	84	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff3, PKC, PLA2, MAPK, PLA2, Ras, CaM, chain, kinetics, PKC, MAPK, Ras, CaM, kinetics[1], PKC, PLA2, MAPK, Ras, CaM, kinetics[2], PKC, PLA2, MAPK, Ras, CaM, kinetics[3], PKC, PLA2, MAPK, Ras, CaM, kinetics[4], PKC, PLA2, MAPK, Ras, CaM, kinetics[5], PKC, PLA2, MAPK, Ras, MAPK, CaM, kinetics[6], PKC, PLA2, MAPK, Ras, CaM, kinetics[7], PKC, PLA2, MAPK, Ras, CaM, PKC, kinetics[8], PLA2, MAPK, Ras, CaM, kinetics[9], PKC, PLA2, MAPK, Ras, CaM, kinetics[10], PKC, PLA2, MAPK, Ras, CaM, kinetics[11], PKC, PLA2, MAPK, Ras, CaM, kinetics[12], PKC, PLA2, Ras, CaM, kinetics[13], PKC, PLA2, MAPK, Ras, CaM, kinetics[14], PKC, PLA2, MAPK, Ras, CaM, kinetics[15], PKC, PLA2, MAPK, Ras, kinetics[16], CaM, PKC, PLA2, MAPK, Ras, CaM, kinetics[17], PKC, PLA2, MAPK, Ras, CaM, kinetics[18], PKC, PLA2, MAPK, Ras, CaM, kinetics[19], PKC, PLA2, MAPK, Ras, CaM, kinetics[20], PKC, PLA2, MAPK, Ras, CaM, kinetics[21], PKC, PLA2, MAPK, Ras, CaM, kinetics[22], PKC, PLA2, MAPK, Ras, CaM, kinetics[23], PKC, PLA2, MAPK, Ras, CaM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times. In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec. The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation. The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

MAPKK* acting as a Molecule in Ajay_Bhalla_2007_ReacDiff3 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992
MAPKK*	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0	125.7	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992

MAPKK* acting as an Enzyme in Ajay_Bhalla_2007_ReacDiff3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
2	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
3	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
4	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
5	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
6	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
7	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
8	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
9	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
10	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
11	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
12	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
13	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
14	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
15	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
16	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
17	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
18	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
19	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
20	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
21	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
22	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
23	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
24	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
25	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
26	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
27	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
28	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
29	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
30	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
31	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
32	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
33	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
34	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
35	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
36	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
37	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
38	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
39	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
40	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
41	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
42	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
43	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
44	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
45	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
46	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
47	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
48	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.
49	MAPKK* / MAPKKtyr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK-tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
50	MAPKK* / MAPKKthr	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.0462964	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
	Rate consts same as for MAPKKtyr.

MAPKK* acting as a Substrate for an Enzyme in Ajay_Bhalla_2007_ReacDiff3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
2	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
3	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
4	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
5	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
6	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
7	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
8	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
9	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
10	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
11	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
12	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
13	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
14	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
15	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
16	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
17	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
18	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
19	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
20	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
21	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
22	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
23	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
24	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
25	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

MAPKK* acting as a Product of an Enzyme in Ajay_Bhalla_2007_ReacDiff3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
3	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
4	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
5	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
6	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
7	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
8	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
9	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
10	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
11	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
12	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
13	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
14	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
15	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
16	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
17	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
18	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
19	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
20	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
21	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
22	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
23	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
24	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
25	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
26	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
27	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
28	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
29	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
30	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
31	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
32	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
33	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
34	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
35	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
36	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
37	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
38	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
39	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
40	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
41	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
42	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
43	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
44	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
45	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
46	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
47	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
48	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
49	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
50	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6

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