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Molecule Parameter List for MAPKK-ser

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MAPKK-ser* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	0	75	75	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff3	84	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff3, PKC, PLA2, MAPK, PLA2, Ras, CaM, chain, kinetics, PKC, MAPK, Ras, CaM, kinetics[1], PKC, PLA2, MAPK, Ras, CaM, kinetics[2], PKC, PLA2, MAPK, Ras, CaM, kinetics[3], PKC, PLA2, MAPK, Ras, CaM, kinetics[4], PKC, PLA2, MAPK, Ras, CaM, kinetics[5], PKC, PLA2, MAPK, Ras, MAPK, CaM, kinetics[6], PKC, PLA2, MAPK, Ras, CaM, kinetics[7], PKC, PLA2, MAPK, Ras, CaM, PKC, kinetics[8], PLA2, MAPK, Ras, CaM, kinetics[9], PKC, PLA2, MAPK, Ras, CaM, kinetics[10], PKC, PLA2, MAPK, Ras, CaM, kinetics[11], PKC, PLA2, MAPK, Ras, CaM, kinetics[12], PKC, PLA2, Ras, CaM, kinetics[13], PKC, PLA2, MAPK, Ras, CaM, kinetics[14], PKC, PLA2, MAPK, Ras, CaM, kinetics[15], PKC, PLA2, MAPK, Ras, kinetics[16], CaM, PKC, PLA2, MAPK, Ras, CaM, kinetics[17], PKC, PLA2, MAPK, Ras, CaM, kinetics[18], PKC, PLA2, MAPK, Ras, CaM, kinetics[19], PKC, PLA2, MAPK, Ras, CaM, kinetics[20], PKC, PLA2, MAPK, Ras, CaM, kinetics[21], PKC, PLA2, MAPK, Ras, CaM, kinetics[22], PKC, PLA2, MAPK, Ras, CaM, kinetics[23], PKC, PLA2, MAPK, Ras, CaM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times. In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec. The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation. The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

MAPKK-ser acting as a Molecule in Ajay_Bhalla_2007_ReacDiff3 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK
MAPKK-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0	125.7	No
Intermediately phophorylated, assumed inactive, form of MAPKK

MAPKK-ser acting as a Substrate for an Enzyme in Ajay_Bhalla_2007_ReacDiff3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
3	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

4	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
5	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
6	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

7	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
8	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
9	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

10	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
11	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
12	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

13	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
14	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
15	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

16	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
17	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
18	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

19	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
20	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
21	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

22	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
23	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
24	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

25	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
26	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
27	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

28	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
29	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
30	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

31	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
32	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
33	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

34	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
35	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
36	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

37	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
38	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
39	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

40	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
41	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
42	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

43	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
44	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
45	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

46	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
47	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
48	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

49	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
50	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
51	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

52	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
53	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
54	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

55	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
56	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
57	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

58	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
59	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
60	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

61	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
62	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
63	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

64	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
65	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
66	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

67	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
68	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
69	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

70	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
71	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
72	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

73	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
74	Raf-GTP-Ras / Raf-GTP-Ras.2	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
75	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

MAPKK-ser acting as a Product of an Enzyme in Ajay_Bhalla_2007_ReacDiff3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 921	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
3	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff3 Pathway No. : 918	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
4	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
5	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 928	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
6	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics Pathway No. : 926	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
7	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
8	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 934	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
9	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[1] Pathway No. : 931	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
10	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
11	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 940	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
12	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[2] Pathway No. : 937	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
13	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
14	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 946	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
15	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[3] Pathway No. : 943	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
16	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
17	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 952	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
18	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[4] Pathway No. : 949	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
19	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
20	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 958	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
21	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[5] Pathway No. : 955	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
22	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
23	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 965	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
24	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[6] Pathway No. : 962	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
25	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
26	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 971	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
27	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[7] Pathway No. : 968	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
28	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
29	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 977	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
30	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[8] Pathway No. : 975	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
31	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
32	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 983	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
33	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[9] Pathway No. : 980	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
34	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
35	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 989	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
36	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[10] Pathway No. : 986	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
37	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
38	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 995	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
39	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[11] Pathway No. : 992	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
40	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
41	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 960	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
42	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[12] Pathway No. : 998	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
43	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
44	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1006	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
45	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[13] Pathway No. : 1003	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
46	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
47	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1012	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
48	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[14] Pathway No. : 1009	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
49	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
50	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1018	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
51	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[15] Pathway No. : 1015	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
52	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
53	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1024	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
54	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[16] Pathway No. : 1020	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
55	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
56	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1030	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
57	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[17] Pathway No. : 1027	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
58	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
59	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1036	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
60	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[18] Pathway No. : 1033	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
61	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
62	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1042	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
63	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[19] Pathway No. : 1039	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
64	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
65	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1048	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
66	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[20] Pathway No. : 1045	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
67	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
68	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1054	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
69	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[21] Pathway No. : 1051	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
70	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
71	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1060	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
72	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[22] Pathway No. : 1057	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
73	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
74	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	MAPK Pathway No. : 1066	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
75	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_ReacDiff3 Accession No. : 84	kinetics[23] Pathway No. : 1063	15.6567	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

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