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Molecule Parameter List for DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
DAG participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences250000750

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff3
    		• 84NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff3,  PKC,  PLA2,  
    MAPK,  PLA2,  Ras,  CaM,  chain,  kinetics,  PKC,  MAPK,  Ras,  CaM,  kinetics[1],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[2],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[3],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[4],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[5],  
    PKC,  PLA2,  MAPK,  Ras,  MAPK,  CaM,  kinetics[6],  PKC,  PLA2,  MAPK,  Ras,  
    CaM,  kinetics[7],  PKC,  PLA2,  MAPK,  Ras,  CaM,  PKC,  kinetics[8],  PLA2,  
    MAPK,  Ras,  CaM,  kinetics[9],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[10],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[11],  PKC,  PLA2,  MAPK,  Ras,  CaM,  
    kinetics[12],  PKC,  PLA2,  Ras,  CaM,  kinetics[13],  PKC,  PLA2,  MAPK,  
    Ras,  CaM,  kinetics[14],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[15],  
    PKC,  PLA2,  MAPK,  Ras,  kinetics[16],  CaM,  PKC,  PLA2,  MAPK,  Ras,  CaM,  
    kinetics[17],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[18],  PKC,  PLA2,  
    MAPK,  Ras,  CaM,  kinetics[19],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[20],  
    PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[21],  PKC,  PLA2,  MAPK,  Ras,  CaM,  
    kinetics[22],  PKC,  PLA2,  MAPK,  Ras,  CaM,  kinetics[23],  PKC,  PLA2,  
    MAPK,  Ras,  CaM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times.
    In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec.
    The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation.
    The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

    DAG acting as a Molecule in      Ajay_Bhalla_2007_ReacDiff3 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3
    Pathway No. : 918
    		• 12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics
    Pathway No. : 926    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[1]
    Pathway No. : 931    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[2]
    Pathway No. : 937    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[3]
    Pathway No. : 943    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[4]
    Pathway No. : 949    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[5]
    Pathway No. : 955    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[6]
    Pathway No. : 962    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[7]
    Pathway No. : 968    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[8]
    Pathway No. : 975    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[9]
    Pathway No. : 980    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[10]
    Pathway No. : 986    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[11]
    Pathway No. : 992    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[12]
    Pathway No. : 998    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[13]
    Pathway No. : 1003    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[14]
    Pathway No. : 1009    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[15]
    Pathway No. : 1015    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[16]
    Pathway No. : 1020    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[17]
    Pathway No. : 1027    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[18]
    Pathway No. : 1033    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[19]
    Pathway No. : 1039    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[20]
    Pathway No. : 1045    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[21]
    Pathway No. : 1051    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[22]
    Pathway No. : 1057    		12.2125.7Yes
    DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• kinetics[23]
    Pathway No. : 1063    		12.2125.7Yes

    DAG acting as a Substrate in a reaction in      Ajay_Bhalla_2007_ReacDiff3 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 919    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    2PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 919    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    3DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 920    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    4PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 927    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    5PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 927    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    6DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 922    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    7PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 932    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    8PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 932    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    9DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 933    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    10PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 938    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    11PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 938    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    12DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 939    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    13PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 944    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    14PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 944    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    15DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 945    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    16PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 950    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    17PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 950    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    18DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 951    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    19PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 956    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    20PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 956    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    21DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 957    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    22PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 963    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    23PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 963    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    24DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 964    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    25PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 969    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    26PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 969    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    27DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 970    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    28PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 974    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    29PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 974    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    30DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 976    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    31PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 981    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    32PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 981    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    33DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 982    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    34PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 987    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    35PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 987    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    36DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 988    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    37PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 993    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    38PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 993    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    39DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 994    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    40PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 999    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    41PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 999    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    42DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1000    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    43PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1004    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    44PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1004    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    45DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1005    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    46PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1010    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    47PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1010    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    48DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1011    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    49PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1016    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    50PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1016    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    51DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1017    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    52PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1022    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    53PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1022    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    54DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1023    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    55PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1028    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    56PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1028    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    57DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1029    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    58PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1034    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    59PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1034    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    60DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1035    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    61PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1040    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    62PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1040    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    63DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1041    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    64PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1046    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    65PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1046    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    66DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1047    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    67PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1052    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    68PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1052    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    69DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1053    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    70PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1058    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    71PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1058    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    72DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1059    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
    73PKC-act-by-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1064    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.4148(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    74PKC-n-DAG
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PKC
    Pathway No. : 1064    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6664(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    75DAG-Ca-PLA2-act
  • Ajay_Bhalla_
    2007_ReacDiff3
    Accession No. : 84
    		• PLA2
    Pathway No. : 1065    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3289(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.


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