| |
Reaction
Name | Pathway Name /
Pathway No. | Kf | Kb | Kd | tau | Reagents |
| 1 | PKC-basal-act | PKC
Pathway No. 364 | 1
(s^-1) | 50
(s^-1) | Keq = 50(uM) | 0.02sec | Substrate:
PKC-cytosolic
Products:
PKC-basal*
|
| Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher. | | 2 | PKC-act-by-DAG | PKC
Pathway No. 364 | 0.008
(uM^-1 s^-1) | 8.6348
(s^-1) | Kd(bf) = 1079.377(uM) | - | Substrate:
DAG
PKC-Ca
Products:
PKC-Ca-DAG
|
| Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8 | | 3 | PKC-Ca-to-memb | PKC
Pathway No. 364 | 1.2705
(s^-1) | 3.5026
(s^-1) | Keq = 2.7569(uM) | 0.21sec | Substrate:
PKC-Ca
Products:
PKC-Ca-memb*
|
| 4 | PKC-n-DAG-AA | PKC
Pathway No. 364 | 0.018
(uM^-1 s^-1) | 2
(s^-1) | Kd(bf) = 111.113(uM) | - | Substrate:
PKC-DAG
AA
Products:
PKC-DAG-AA
|
| Reduced kf to 0.66X to match Shinomura et al data. Initial: kf = 3.3333e-9 New: 2e-9 Newer: 2e-8 kb was 0.2 now 2. | | 5 | PKC-act-by-Ca | PKC
Pathway No. 364 | 0.6
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.8333(uM) | - | Substrate:
PKC-cytosolic
Ca
Products:
PKC-Ca
|
| Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5 | | 6 | PKC-act-by-DAG-A
A | PKC
Pathway No. 364 | 2
(s^-1) | 0.2
(s^-1) | Keq = 0.1(uM) | 0.455sec | Substrate:
PKC-DAG-AA
Products:
PKC-DAG-AA*
|
| Assume slowish too. Schaechter and Benowitz | | 7 | PKC-DAG-to-memb | PKC
Pathway No. 364 | 1
(s^-1) | 0.1
(s^-1) | Keq = 0.1(uM) | 0.909sec | Substrate:
PKC-Ca-DAG
Products:
PKC-DAG-memb*
|
| Raise kb from .087 to 0.1 to match data from Shinomura et al. Lower kf from 1.155 to 1.0 to match data from Shinomura et al. | | 8 | PKC-act-by-Ca-AA | PKC
Pathway No. 364 | 0.0012
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 83.3326(uM) | - | Substrate:
PKC-Ca
AA
Products:
PKC-Ca-AA*
|
| Schaechter and Benowitz We have to increase Kf for conc scaling Changed kf to 2e-9 on Sept 19, 94. This gives better match. | | 9 | PKC-act-by-AA | PKC
Pathway No. 364 | 0.0001
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 833.3264(uM) | - | Substrate:
AA
PKC-cytosolic
Products:
PKC-AA*
|
| Raise kf from 1.667e-10 to 2e-10 to get better match to data. | | 10 | PKC-n-DAG | PKC
Pathway No. 364 | 0.0006
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 166.6656(uM) | - | Substrate:
PKC-cytosolic
DAG
Products:
PKC-DAG
|
| kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9 |
and 
color.
