| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
craf**-deph
Enzyme Molecule:
PPhosphatase2A | 15.6566 | 6 | 4.16667 | explicit E-S complex | craf-1**
| craf-1*
|
| | Ueki et al JBC 269(22) pp 15756-15761 1994 show hyperphosphorylation of craf, so this is there to dephosphorylate it. Identity of phosphatase is not known to me, but it may be PP2A like the rest, so I have made it so. |
| 2 |
Enzyme Activity:
craf-deph
Enzyme Molecule:
PPhosphatase2A | 15.6566 | 6 | 4.16667 | explicit E-S complex | craf-1*
| craf-1
|
| | See parent PPhosphatase2A for parms |
| 3 |
Enzyme Activity:
MAPK*
Enzyme Molecule:
MAPK* | 25.641 | 20 | 4 | explicit E-S complex | PLA2-cytosolic
| PLA2*
|
| | Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5 |
| 4 |
Enzyme Activity:
MAPK*-feedback
Enzyme Molecule:
MAPK* | 25.641 | 10 | 4 | explicit E-S complex | craf-1*
| craf-1**
|
| | Ueki et al JBC 269(22):15756-15761 show the presence of this step, but not the rate consts, which are derived from Sanghera et al JBC 265(1):52-57, 1990, see the deriv in the MAPK* notes. |
| 5 |
Enzyme Activity:
MAPKK-deph
Enzyme Molecule:
PPhosphatase2A | 15.6566 | 6 | 4.16667 | explicit E-S complex | MAPKK*
| MAPKK-ser
|
| | See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms. |
| 6 |
Enzyme Activity:
MAPKK-deph-ser
Enzyme Molecule:
PPhosphatase2A | 15.6566 | 6 | 4.16667 | explicit E-S complex | MAPKK-ser
| MAPKK
|
| 7 |
Enzyme Activity:
MKP-1-phosph
Enzyme Molecule:
MAPK* | 25.641 | 1 | 4 | explicit E-S complex | MKP-1
| MKP-1-ser359*
|
| | 3 Feb 2000. See Brondello et al Science 286:2514 1999. Rates assumed standard MAPK rates based on Sanghera et al JBC 265(1):53-57 1990. 24 Apr 2K, based on 12 Feb 2K: scaled by 0.1 to 3.25e-7,4,1 |
| 8 |
Enzyme Activity:
MKP-1-phosph2
Enzyme Molecule:
MAPK* | 25.641 | 1 | 4 | explicit E-S complex | MKP-1-ser359*
| MKP-1**
|
| | 3 Feb 2000. See Brondello et al Science 286:2514 1999. Rates assumed standard MAPK rates based on Sanghera et al JBC 265(1):53-57 1990. 24 Apr 2K, based on 12 Feb 2K: Scaled x0.1 to 3.25e-7,4,1 |
| 9 |
Enzyme Activity:
MKP1-thr-deph
Enzyme Molecule:
MKP-1 | 0.0666667 | 1 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MAPK*
| MAPK-tyr
|
| | See MKP1-tyr-deph |
| 10 |
Enzyme Activity:
MKP1-tyr-deph
Enzyme Molecule:
MKP-1 | 0.0666667 | 1 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MAPK-tyr
| MAPK
|
| | The original kinetics have been modified to obey the k2 = 4 * k3 rule, while keeping kcat and Km fixed. As noted in the NOTES, the only constraining data point is the time course of MAPK dephosphorylation, which this model satisfies. It would be nice to have more accurate estimates of rate consts and MKP-1 levels from the literature. Effective Km : 67 nM kcat = 1.43 umol/min/mg |
| 11 |
Enzyme Activity:
PKC-act-GEF
Enzyme Molecule:
PKC-active | 3.33333 | 4 | 4 | explicit E-S complex | inact-GEF
| GEF*
|
| | Rate consts from PKC-act-raf. This reaction activates GEF. Gives >= 2X stim of ras, and a 2X stim of MAPK over amount from direct phosph of c-raf. Note that it is a push-pull reaction, and also get eff through phosph and inact of GAPs. |
| 12 |
Enzyme Activity:
PKC-act-raf
Enzyme Molecule:
PKC-active | 66.6667 | 4 | 4 | explicit E-S complex | craf-1
| craf-1*
|
| | Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 Lower k1 to 1e-6 to balance 10X DAG sensitivity of PKC 11 May2K: Double Vmax. rates=1e-6, 32,8 29 May 2k: Vmax * 0.6. Rates=6e-7, 19.2, 4.8 30 May 2k: vmax=5. Rates=6.25e-7,20,5 3 Jun 2k: vmax=4. Rates=5e-7,16,4 |
| 13 |
Enzyme Activity:
PKC-inact-GAP
Enzyme Molecule:
PKC-active | 3.33333 | 4 | 4 | explicit E-S complex | GAP
| GAP*
|
| | Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review. |
