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Enzyme List for pathway EGFR (Pathway Number 77) | | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
phosph_PLC_g
Enzyme Molecule:
L.EGFR | 0.333333 | 0.2 | 4 | explicit E-S complex | Ca.PLC_g
| Ca.PLC_g*
| | | Hsu et al JBC 266:1 603-608 1991 Km = 385 +- 100 uM, Vm = 5.1 +-1 pmol/min/ug for PLC-771. Other sites have similar range, but are not stim as much by EGF. These rates are improbably slow and are for the intracellular domain of EGFR alone. Wahl et al JBC 267(15) 10447-10456 1992 reports a tau of 5min for activation of PLC-gamma in vivo. Also Sherrill and Kyte say turnover # for angiotensin II is 5/min for cell extt, and 2/min for placental. Also see Okada et al JBC 270(35) 20737-20741 1995 for Shc rates where Km = 0.7 and Vmax = 4.4 pmol/min To achieve these turnovers and time-courses, we may need to consider the membrane-plane action of the the receptor, but for now the rates are consistent with the Wahl et al reports. |
| 2 |
Enzyme Activity:
phosph_Shc
Enzyme Molecule:
L.EGFR | 0.833333 | 0.2 | 4 | explicit E-S complex | SHC
| SHC*
| | | Rates from Okada et al JBC 270:35 pp 20737 1995 Km = 0.70 to 0.85 uM, Vmax = 4.4 to 5.0 pmol/min. Unfortunately the amount of enzyme is not known, the prep is only partially purified. Time course of phosph is max within 30 sec, falls back within 20 min. Ref: Sasaoka et al JBC 269:51 32621 1994. Use k3 = 0.1 based on this tau. |
Pathway Details Molecule List Enzyme List Reaction List
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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