| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | CaMCa2-CANAB | 0 | 1000 | No |
| | Ca2.CaM.Ca4.CaNAB The CaM has only 2 calciums attached, but CaNAB has all 4. This contributes equally to enzyme activity along with the Ca3 and Ca4 forms. |
| 2 | CaMCa3-CaNAB | 0 | 1000 | No |
| | Ca3.CaM.Ca4.CaNAB This is assumed to have the same enzymatic activity as the other Ca states of CaM bound to CaNAB. Hence I just put this into a summation with the other states and utilize the sum for the enzymatic steps. |
| 3 | CaMCa4-CaNAB | 0 | 1000 | No |
| | Ca4.CaM.Ca4.CaNAB. This form is fullly loaded with Ca. Its activity is assumed to be the same as the lower Ca-bound forms of CaM.Ca4.CaNAB. |
| 4 | CaNAB | 1 | 1000 | No |
| | Calcineurin A and B subunits. We assume that the A and B subunits of PP2B are always bound under physiological conditions. Tallant and Cheung '83 Biochem 22 3630-3635 have conc in many species, average for mammalian brain is around 1 uM. |
| 5 | CaNAB-Ca2 | 0 | 1000 | No |
| | Form of CaN with two Calciums bound. |
