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Reaction List for pathway EGFR (Pathway Number 77) | | Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| | Name | Kf | Kb | Kd | tau | Substrate | Product | | 1 |
act_EGFR | 4.2
(uM^-1 s^-1) | 0.25
(s^-1) | Kd(bf) = 0.0595(uM) | - | EGFR
EGF
| L.EGFR
| | | Affinity of EGFR for EGF is complex: depends on [EGFR]. We'll assume fixed [EGFR] and use exptal affinity ~20 nM (see Sherrill and Kyte Biochem 1996 35 5705-5718, Berkers et al JBC 266:2 922-927 1991, Sorokin et al JBC 269:13 9752-9759 1994). Tau =~2 min (Davis et al JBC 263:11 5373-5379 1988) or Berkers Kass = 6.2e5/M/sec, Kdiss=3.5e-4/sec. Sherrill and Kyte have Hill Coeff=1.7 | | 2 |
dephosph_Shc | 0.0017
(s^-1) | 0
(s^-1) | - | - | SHC*
| SHC
| | | Time course of decline of phosph is 20 min from Sasaoka et al 1994 JCB 269(51):32621. Part of this is the turnoff time of the EGFR itself. Lets assume a tau of 10 min for this dephosph, which means a Kf of 0.0016667 | | 3 |
Internalize | 0.002
(s^-1) | 0.0003
(s^-1) | Keq = 0.165(uM) | 434.783sec | L.EGFR
| Internal_L.EGFR
| | | See Helin and Beguinot JBC 266:13 1991 pg 8363-8368. In Fig 3 they have internalization tau about 10 min, equil at about 20% EGF available. So kf = 4x kb, and 1/(kf + kb) = 600 sec so kb = 1/3K = 3.3e-4, and kf = 1.33e-3. This doesn't take into account the unbound receptor, so we need to push the kf up a bit, to 0.002 This reaction is treated as reversible, thereby making the approximation that the re-insertion of EGFR into the membrane is handled by the same process going backwards. |
Pathway Details Molecule List Enzyme List Reaction List
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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