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Molecule Parameter List for IP5(13456) | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Osc_Ca_
IP3metabolism | 32 | Network |
MIPP, CaMKII, CaM,
PKC, IP3-3K, Gq,
PLCbeta, 134_dephos, 145_dephos,
IP4-system, IHP-system, 1345_dephos,
CaRegulation, Othmer-Tang-model | | This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. |
IP5(13456) acting as a Molecule in Osc_Ca_IP3metabolism Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | IP5(13456) | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP4-system
Pathway No. : 167 | 53.9992 | 1000 | No | | Inositol(13456)pentakisphosphate Conc from Voglmaier et al, PNAS 93; 1996 |
IP5(13456) acting as a Substrate for an Enzyme in Osc_Ca_IP3metabolism Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | IP6_K2 /
ip5_k2 | Osc_Ca_
IP3metabolism
Accession No. : 32 | IHP-system
Pathway No. : 168 | 8.4 | 0.0572 | 4 | explicit E-S complex | Substrate
IP5(13456)
Product
PP-IP4
| | | from Saiardi et al, JBC 275(32); 2000: 24686-92 | | 2 | IP6-K /
ip5_k1 | Osc_Ca_
IP3metabolism
Accession No. : 32 | IHP-system
Pathway No. : 168 | 6.69999 | 0.234 | 4 | explicit E-S complex | Substrate
IP5(13456)
Product
PP-IP4
| | | from Saiardi et al, JBC 275(32); 2000: 24686-92 |
IP5(13456) acting as a Substrate in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | IP5-dipp-inhib | Osc_Ca_
IP3metabolism
Accession No. : 32 | IHP-system
Pathway No. : 168 | 1
(uM^-1 s^-1) | 1.6
(s^-1) | Kd(bf) = 1.6(uM) | - | Substrate
DIPP1
IP5(13456)
Product
IP5-DIPPcmplx
| | | IC50=1.6uM from Safrany et al, EMBO J 17(22); 1998 | | 2 | IP5-inhib-5pase | Osc_Ca_
IP3metabolism
Accession No. : 32 | 145_dephos
Pathway No. : 166 | 1
(uM^-1 s^-1) | 45
(s^-1) | Kd(bf) = 44.9991(uM) | - | Substrate
IP5(13456)
IP_5pase1
Product
IP5-5pase-cmplx
| | | from Hoer and Oberdisse, Biochem J 278; 1991: 219-224 | | 3 | ip5_3pase | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP4-system
Pathway No. : 167 | 0.0003
(s^-1) | 0
(s^-1) | - | - | Substrate
IP5(13456)
Product
IP4(1456)
| | | Ins(13456)P5 3-phosphatase from Nogimori et al, JBC 266; 1991 Backflux from the reversible InsP4 3-kinase is not sufficient to maintain Ins(1456)P4 levels. Hence this reaction is necessary. This reaction also maintains the flux in the network. | | 4 | ip5_1pase | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP4-system
Pathway No. : 167 | 0.0136
(s^-1) | 0
(s^-1) | - | - | Substrate
IP5(13456)
Product
IP4(3456)
| | | InsP5 1-phosphatase Reaction is necessary to generate the flux of Ins(3456)P4 seen physiologically (Ho et al Curr Biol 2002, 12:1-20), although the IP4-1K/pase can maintain basal levels of this InsP4. | | 5 | ip5-kinase-pase | Osc_Ca_
IP3metabolism
Accession No. : 32 | IHP-system
Pathway No. : 168 | 1
(s^-1) | 1.92
(s^-1) | Keq = 1.92(uM) | 0.342sec | Substrate
IP5(13456)
Product
IP6
| | | Kf represents InsP5 2-kinase and Kb represents InsP6 2-phosphatase Although InsP5 2-kinases in yeast and plant systems have been characterized (Ives et al, JBC 275; 2000: 36575-83), the mammalian counterpart is still to be worked out. Rates calculated to maintain InsP5 and InsP6 levels | | 6 | IP5-inhib-3pase | Osc_Ca_
IP3metabolism
Accession No. : 32 | 1345_dephos
Pathway No. : 169 | 1
(uM^-1 s^-1) | 0.06
(s^-1) | Kd(bf) = 0.06(uM) | - | Substrate
1345_3pase
IP5(13456)
Product
IP5-3pase-cmplx
| | | from Hoer and Oberdisse, Biochem J 278; 1991: 219-224 |
IP5(13456) acting as a Product in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | ip5(13456)_trp | Osc_Ca_
IP3metabolism
Accession No. : 32 | MIPP
Pathway No. : 158 | 1
(s^-1) | 0.001
(s^-1) | Not applicable** | - | Substrate
IP5(13456)_ER
Product
IP5(13456)
| | | Ins(13456)P5 ER-cytosol transport | | 2 | ip4-5K | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP4-system
Pathway No. : 167 | 0.15
(s^-1) | 0.003
(s^-1) | Keq = 0.02(uM) | 6.536sec | Substrate
IP4(1346)
Product
IP5(13456)
| | | Kf based on basal levels of Ins(1346)P4 Kb obtained from dG calculations for equilibrium product substrate conditions. | | 3 | ip4-3k-off | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP4-system
Pathway No. : 167 | 4.3
(s^-1) | 0.2687
(uM^-1 s^-1) | Kd(cb) = 0.0625(uM) | - | Substrate
ip4_3k_cmplx
Product
IP4-3K
IP5(13456)
| | | Kf = Vmax for enzyme: Stephens et al, Biochem J 249; 1988. Kb ascertained from dG calculations for equilibrium conditions. | | 4 | ip4-1k-off | Osc_Ca_
IP3metabolism
Accession No. : 32 | IP4-system
Pathway No. : 167 | 0.624
(s^-1) | 0.056
(uM^-1 s^-1) | Kd(cb) = 0.0897(uM) | - | Substrate
ip4_1k_cmplx
Product
IP3-56K_IP4-1K
IP5(13456)
| | | Kf = enzyme Vmax : Yang and Shears, Biochem J 2000, 351, 551-5. Kb ascertained from dG calculations for equilibrium product substrate conditions. Ho et al, Curr Biol 2002, 12: 1-20 report a Vmax for reverse reaction of 0.0656 /sec. But this cannot be exactly incorporated unless Km for InsP5 is also known. Simulations show that incorporation of this reverse reaction can maintain basal InsP4 levels but cannot achieve the stimulated InsP4 levels shown in Ho et al. Hence the separate InsP5 1pase is retained. | | 5 | dipp_ip6 | Osc_Ca_
IP3metabolism
Accession No. : 32 | IHP-system
Pathway No. : 168 | 0
(s^-1) | 0
(s^-1) | - | - | Substrate
PP-IP4
Product
IP5(13456)
| | | rate based on basal levels of PP-InsP4 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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