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Molecule Parameter List for MKP-1**

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MKP-1** participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1120120

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • mkp1_feedback_
    effects
  • 4Network
    Shared_Object_mkp1_feedback_effects Sos PKC 
    MAPK PLA2 Ras 
    PDGFR 
    This is a network involving the MAPK-PKC feedback loop with input from the PDGFR in the synapse. The distinctive feature of this model is that it includes MKP-1 induction by MAPK, and the consequent inhibitory regulation of MAPK and the feedback loop. Lots of interesting dynamics arise from this. This link provides supplementary material for the paper Bhalla US et al. Science (2002) 297(5583):1018-23. In the form of several example simulations and demos for the figures in the paper.

    MKP-1** acting as a Molecule in  
    mkp1_feedback_effects Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    MKP-1**
  • mkp1_feedback_
    effects

    Accession No. : 4
  • MAPK
    Pathway No. : 35
    01000No
    Same enzyme activity as MKP-1, but different degradation rates. Brondello et al Science 286:2514 1999 This form is doubly phosphorylated.

    MKP-1** acting as a Summed Molecule in  
    mkp1_feedback_effects Network
    Accession NamePathway NameTargetInput
  • mkp1_feedback_
    effects

    Accession No. : 4
  • Shared_Object_
    mkp1_feedback_
    effects

    Pathway No. : 32
  • tot_MKP1MKP-1
    MKP-1-ser359*
    MKP-1**
    Total available active MKP-1. This sums the levels of the non-phosphorylated, singly phosphorylated and doubly phosphorylated forms. It is used primarily for graphing the total MKP-1 level.

    MKP-1** acting as an Enzyme in  
    mkp1_feedback_effects Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1MKP-1** /
    MKP1*-tyr-deph
  • mkp1_feedback_
    effects

    Accession No. : 4
  • MAPK
    Pathway No. : 35
    0.066666714Classical Michaelis-Menten
    V = Etot.S.Kcat/Km+S
    Substrate
    MAPK-tyr

    Product
    MAPK
        3 Feb 2000. Same rates as MKP-1.
    2MKP-1** /
    MKP1*-thr-deph
  • mkp1_feedback_
    effects

    Accession No. : 4
  • MAPK
    Pathway No. : 35
    0.066666714Classical Michaelis-Menten
    V = Etot.S.Kcat/Km+S
    Substrate
    MAPK*

    Product
    MAPK-tyr
        3 Feb 2000. Same rates as MKP1

    MKP-1** acting as a Product of an Enzyme in  
    mkp1_feedback_effects Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MAPK*  /
    MKP-1-phosph2
  • mkp1_feedback_
    effects

    Accession No. : 4
  • Shared_Object_
    mkp1_feedback_
    effects

    Pathway No. : 32
  • 25.64114explicit E-S complexSubstrate
    MKP-1-ser359*

    Product
    MKP-1**
    3 Feb 2000. MAPK rates based on Sanghera JS, Paddon HB, Bader SA, Pelech SL. (1990) 265(1):52-57. The Vmax is scaled down 10 fold to match the time-course of phosph by MAPK, from Brondello JM, Pouyssegur J, McKenzie FR. (1999) Science 286(5449):2514-2517.

    MKP-1** acting as a Substrate in a reaction in  
    mkp1_feedback_effects Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1turnover_MKP1**
  • mkp1_feedback_
    effects

    Accession No. : 4
  • MAPK
    Pathway No. : 35
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    MKP-1**

    Product
    Ubiquitination
      Rate of turnover of phosph form of MKP1 is from Brondello et al Science 286:2514 1999. Tau is about 150 min though it is not a clean exponential falloff.
    2MKP-1**dephosph
  • mkp1_feedback_
    effects

    Accession No. : 4
  • MAPK
    Pathway No. : 35
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    MKP-1**

    Product
    MKP-1-ser359*
      24 Apr 2K, based on 12 Feb 2K: Scaled up 10x to kf=0.01, kb=0 13 Apr 2001. Slowing down to 0.0001 to match turnover rates.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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