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Molecule Parameter List for PA.Ca.PLCbeta

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PA.Ca.PLCbeta* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	0	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
PLCbeta1	13	Pathway	PLCbeta
This is a model of PLCbeta1 in the bovine brain membranes as stimulated by Ca, Gq and PA. The primary data sources are Irene Litosch Biochemistry (2000) 39(26):7736-43, Sternweis PC et al. Philos Trans R Soc Lond B Biol Sci. (1992) 336(1276):35-41 and Smrcka AV et al. Science (1991) 251(4995):804-7.

PA.Ca.PLCbeta acting as a Molecule in PLCbeta1 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PA.Ca.PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	0	0.0016667	No
6 Nov 2001 This is the active enzyme complex formed by binding of PA with Ca.PLCbeta.All the activity of PA is possible only in the presence of Calcium bound enzyme i.e., Ca.PLCbeta and resulted in dose response curves for the activity obtained by I. Litosch., Biochem., 2000, 39, 7736-7743.

PA.Ca.PLCbeta acting as an Enzyme in PLCbeta1 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PA.Ca.PLCbeta / PA.Ca.PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	10	38	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate PIP2 Product DAG IP3
3 Nov 2001 The Km and Vmax were fixed relatively to the Km and Vmax values of Ca.PLCbeta and Gq.Ca.PLCbeta which were obtained from the works of Sternweis et al and Smrcka et al where the Km varied between 5 and 20uM and Vmax between 7 and 23 umol/min/mg depending on the presence and absence of Gq.

PA.Ca.PLCbeta acting as a Product in a reaction in PLCbeta1 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PA-bind-Ca.PLCbe ta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	1 (uM^-1 s^-1)	8 (s^-1)	Kd(bf) = 8(uM)	-	Substrate Ca.PLCbeta PA Product PA.Ca.PLCbeta
6 Nov 2001 The Kf and Kb values were fixed according to the dose response curves obtained from I. Litosch, Biochem., 2000, 39, 7736-7743.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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