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Molecule Parameter List for IP4(1345)

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

IP4(1345) participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	2	5	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Osc_Ca_ IP3metabolism	24	Network	MIPP,  CaMKII,  CaM,   PKC,  IP3-3K,  Gq,   PLCbeta,  134_dephos,  145_dephos,   IP4-system,  IHP-system,  1345_dephos,   CaRegulation,  Othmer-Tang-model
This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

IP4(1345) acting as a Molecule in  Osc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
IP4(1345)	Osc_Ca_ IP3metabolism Accession No. : 24	IP3-3K Pathway No. : 124	1	1000	No
Inositol(1345)tetrakisphosphate

IP4(1345) acting as a Substrate for an Enzyme in  Osc_Ca_IP3metabolism Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	IP_5pase1  / ip4_5pase	Osc_Ca_ IP3metabolism Accession No. : 24	145_dephos Pathway No. : 128	0.80003	0.09	4	explicit E-S complex	Substrate IP4(1345) Product IP3(134)
	Ins(1345)P4 5-phosphatase activity of InsP 5-phosphatase1 from Hansen et al, JBC 262; 1987 vmax for IP4 substrate is approx 0.1 times vmax for IP3
2	1345_3pase  / ip4_3pase	Osc_Ca_ IP3metabolism Accession No. : 24	1345_dephos Pathway No. : 131	0.400007	0.0012	4	explicit E-S complex	Substrate IP4(1345) Product IP3(145)
	Ins(1345)P4 3-phosphatase from Nogimori et al, JBC 266; 1991

IP4(1345) acting as a Product of an Enzyme in  Osc_Ca_IP3metabolism Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	IP3_3K  / ip3-3k	Osc_Ca_ IP3metabolism Accession No. : 24	IP3-3K Pathway No. : 124	1.40001	3.0475	4	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Johanson et al, JBC 263; 1988 Original Vmax scaled up by 50% to obtain value at 37C, as enzyme assay was done at 30C Km increased from 0.2 to 1.4 as per various other reports (Shears Review, BiochemJ 260; 1989)
2	IP3_3K*1  / ip3-3k*1	Osc_Ca_ IP3metabolism Accession No. : 24	IP3-3K Pathway No. : 124	1.40004	0.76187	4.00003	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Sim et al, JBC 265; 1990
3	IP3_3K*  / ip3-3k*	Osc_Ca_ IP3metabolism Accession No. : 24	IP3-3K Pathway No. : 124	1.40001	3.0475	4	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Communi et al, EMBO J 16; 1997 In absence of CaM binding, activity same as that of non-phosphorylated enzyme
4	IP3_3K_CaM*  / ip3-3k-CaM*	Osc_Ca_ IP3metabolism Accession No. : 24	IP3-3K Pathway No. : 124	2.80002	45	4	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Communi et al, EMBO J 16; 1997 Vmax adjusted to obtain 9-fold greater activity than ip3-3k
5	IP3_3K_CaM  / ip3-3k-CaM	Osc_Ca_ IP3metabolism Accession No. : 24	IP3-3K Pathway No. : 124	2.8001	10.5	4.0001	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Erneux et al, Biochem 214; 1993 2-2.5 fold more active than ip3-3k but Km is doubled, hence Vmax adjusted accordingly.

IP4(1345) acting as a Substrate in a reaction in  Osc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	ip4(1345)_trp	Osc_Ca_ IP3metabolism Accession No. : 24	MIPP Pathway No. : 120	0.001 (s^-1)	1 (s^-1)	Not applicable**	-	Substrate IP4(1345) Product IP4(1345)_ER
	Ins(1345)P4 ER-cytosol transport
2	ip4-inhib-56k[1]	Osc_Ca_ IP3metabolism Accession No. : 24	IP4-system Pathway No. : 129	1 (uM^-1 s^-1)	6.3 (s^-1)	Kd(bf) = 6.3(uM)	-	Substrate IP3-56Kcmplx IP4(1345) Product IP4(1345)-56k-cm plx
	from Shears, JBC 264(33); 1989: 19879-86

IP4(1345) acting as a Product in a reaction in  Osc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
5kinase	Osc_Ca_ IP3metabolism Accession No. : 24	IP4-system Pathway No. : 129	0.0132 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate IP3-56Kcmplx Product IP3-56K IP4(1345)
Kf = 0.274 times Vmax of IP3 56-K as product ratio of Ins(1345)P4 : Ins(1346)P4 is 1 : 2.3-5 from Wilson and Majerus, JBC 271; 1996