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Result: 1 - 20 of 70 rows are displayed Previous of 4  Next

Reaction List for Accession fig4_synapse (Accession Number 3)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name		Pathway Name / 
Pathway No.		KfKbKdtauReagents
1 remove_glu
  • Shared_Object_
    fig4_synapse
    Pathway No. 19
    		• 500
    (s^-1)    		1000
    (s^-1)    		Keq = 2(uM)0.001sec  Substrate:
     Glu

     Products:
     synapse
    2 
  • cAMP-bind-site-A
    1
    		• PKA

    Pathway No. 30    		75
    (uM^-1 s^-1)    		110
    (s^-1)    		Kd(bf) = 1.4667(uM)-  Substrate:
     R2C2-cAMP2
     cAMP

     Products:
     R2C2-cAMP3
     Kf = 75 /sec/uM, Kb = 110 /sec; This site has higher Kd for cAMP (kinetics within bovine myocardium)
    Dagfinn Ogreid and Stein Ove Doskeland (1981) FEBS Lett. 129(2):287-292
    3 CaM-TR2-bind-CaCaM

    Pathway No. 27    		72
    (uM^-2 s^-1)    		72
    (s^-1)    		Kd(af) = 1(uM)-  Substrate:
     CaM
     Ca
     Ca

     Products:
     CaM-TR2-Ca2
    4 PKC-basal-actPKC

    Pathway No. 20    		1
    (s^-1)    		50
    (s^-1)    		Keq = 50(uM)0.02sec  Substrate:
     PKC-cytosolic

     Products:
     PKC-basal*
    5 
  • cAMP-bind-site-B
    1
    		• PKA

    Pathway No. 30    		54
    (uM^-1 s^-1)    		33
    (s^-1)    		Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2
     cAMP

     Products:
     R2C2-cAMP
     Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741
    Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595
    Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7
    6 
  • cAMP-bind-site-B
    2
    		• PKA

    Pathway No. 30    		54
    (uM^-1 s^-1)    		33
    (s^-1)    		Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2-cAMP
     cAMP

     Products:
     R2C2-cAMP2
     Kf = 54 /sec/uM, Kb = 33 /sec; Same Km (1e-07M) assumed as site B1.
    kf/kb = 0.7e-07M*1e06/(6e05^02) = 2e-13:2.77e-12
    7 
  • cAMP-bind-site-A
    2
    		• PKA

    Pathway No. 30    		75
    (uM^-1 s^-1)    		32.5
    (s^-1)    		Kd(bf) = 0.4333(uM)-  Substrate:
     cAMP
     R2C2-cAMP3

     Products:
     R2C2-cAMP4
     Now cAMP shows effects of cooperativity and PKA has a low Kd for cAMP.
    8 Release-C1PKA

    Pathway No. 30    		60
    (s^-1)    		18
    (uM^-1 s^-1)    		Kd(cb) = 0.3(uM)-  Substrate:
     R2C2-cAMP4

     Products:
     PKA-active
     R2C-cAMP4
     The complex starts to dissociate and release catalytic subunit C. This has to be fast, as the activation of PKA by cAMP is also fast.
    9 Release-C2PKA

    Pathway No. 30    		60
    (s^-1)    		18
    (uM^-1 s^-1)    		Kd(cb) = 0.3(uM)-  Substrate:
     R2C-cAMP4

     Products:
     PKA-active
     R2-cAMP4
    10 
  • RecLigandBinding
    		• Gq

    Pathway No. 23    		16.8
    (uM^-1 s^-1)    		10
    (s^-1)    		Kd(bf) = 0.5952(uM)-  Substrate:
     mGluR
     Glu

     Products:
     Rec-Glu
    11 
  • CaM-TR2-Ca2-bind
    -Ca
    		• CaM

    Pathway No. 27    		3.6
    (uM^-1 s^-1)    		10
    (s^-1)    		Kd(bf) = 2.7778(uM)-  Substrate:
     CaM-TR2-Ca2
     Ca

     Products:
     CaM-Ca3
    12 CaM-Ca3-bind-CaCaM

    Pathway No. 27    		0.465
    (uM^-1 s^-1)    		10
    (s^-1)    		Kd(bf) = 21.5054(uM)-  Substrate:
     CaM-Ca3
     Ca

     Products:
     CaM-Ca4
    13 PKC-act-by-DAGPKC

    Pathway No. 20    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.377(uM)-  Substrate:
     DAG
     PKC-Ca

     Products:
     PKC-Ca-DAG
    14 CaMKII-bind-CaMCaMKII

    Pathway No. 26    		49.9998
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.1(uM)-  Substrate:
     CaM-Ca4
     CaMKII

     Products:
     CaMKII-CaM
    15 CaM_bind_PDE1AC

    Pathway No. 31    		720
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.0069(uM)-  Substrate:
     PDE1
     CaM-Ca4

     Products:
     CaM.PDE1
    16 DAG-Ca-PLA2-actPLA2

    Pathway No. 21    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3333(uM)-  Substrate:
     DAG
     PLA2-Ca*

     Products:
     DAG-Ca-PLA2*
    17 PKC-Ca-to-membPKC

    Pathway No. 20    		1.2705
    (s^-1)    		3.5026
    (s^-1)    		Keq = 2.7569(uM)0.21sec  Substrate:
     PKC-Ca

     Products:
     PKC-Ca-memb*
    18 PKC-n-DAG-AAPKC

    Pathway No. 20    		0.018
    (uM^-1 s^-1)    		2
    (s^-1)    		Kd(bf) = 111.1111(uM)-  Substrate:
     PKC-DAG
     AA

     Products:
     PKC-DAG-AA
    19 Act-PLC-CaPLCbeta

    Pathway No. 22    		3
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.3333(uM)-  Substrate:
     Ca
     PLC

     Products:
     PLC-Ca
    20 Act-PLC-by-GqPLCbeta

    Pathway No. 22    		25.2
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0397(uM)-  Substrate:
     G*GTP
     PLC-Ca

     Products:
     PLC-Ca-Gq
     
    Result: 1 - 20 of 70 rows are displayed Previous of 4  Next


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