| |
Reaction
Name | Pathway Name /
Pathway No. | Kf | Kb | Kd | tau | Reagents |
| 1 | CaM-bind-Ca | CaM
Pathway No. 173 | 1
(uM^-1 s^-1) | 20
(s^-1) | Kd(bf) = 19.9992(uM) | - | Substrate:
CaM
Ca
Products:
CaMCa
|
| 2 | CaMCa-bind-Ca | CaM
Pathway No. 173 | 33.2998
(uM^-1 s^-1) | 20
(s^-1) | Kd(bf) = 0.6006(uM) | - | Substrate:
CaMCa
Ca
Products:
CaMCa2
|
| 3 | CaMCa2-bind-Ca | CaM
Pathway No. 173 | 5
(uM^-1 s^-1) | 500
(s^-1) | Kd(bf) = 99.9994(uM) | - | Substrate:
CaMCa2
Ca
Products:
CaMCa3
|
| 4 | CaMCa3-bind-Ca | CaM
Pathway No. 173 | 100.004
(uM^-1 s^-1) | 500
(s^-1) | Kd(bf) = 4.9998(uM) | - | Substrate:
CaMCa3
Ca
Products:
CaMCa4
|
| 5 | CaMKII-CaMCa-bin
d-Ca | CaMKII
Pathway No. 174 | 100.004
(uM^-1 s^-1) | 20
(s^-1) | Kd(bf) = 0.2(uM) | - | Substrate:
CAMKII-CaMCa
Ca
Products:
CaMKII-CaMCa2
|
| 6 | CaMKII-bind-CaM | CaMKII
Pathway No. 174 | 0.2
(uM^-1 s^-1) | 2700
(s^-1) | Kd(bf) = 13500(uM) | - | Substrate:
CaM
CaMKII
Products:
CaMKII-CaM
|
| 7 | CaMKII-bind-CaMC
a | CaMKII
Pathway No. 174 | 0.8
(uM^-1 s^-1) | 2700
(s^-1) | Kd(bf) = 3375.0084(uM) | - | Substrate:
CaMCa
CaMKII
Products:
CAMKII-CaMCa
|
| 8 | CaMKII-bind-CaMC
a2 | CaMKII
Pathway No. 174 | 2
(uM^-1 s^-1) | 2250
(s^-1) | Kd(bf) = 1125(uM) | - | Substrate:
CaMCa2
CaMKII
Products:
CaMKII-CaMCa2
|
| 9 | CaMKII-bind-CaMC
a3 | CaMKII
Pathway No. 174 | 100.004
(uM^-1 s^-1) | 22.5
(s^-1) | Kd(bf) = 0.225(uM) | - | Substrate:
CaMCa3
CaMKII
Products:
CaMKII-CaMCa3
|
| 10 | CaMKII-CaMCa2-bi
nd-Ca | CaMKII
Pathway No. 174 | 100.004
(uM^-1 s^-1) | 2
(s^-1) | Kd(bf) = 0.02(uM) | - | Substrate:
CaMKII-CaMCa2
Ca
Products:
CaMKII-CaMCa3
|
| 11 | CaMKII-CaMCa3-bi
nd-Ca | CaMKII
Pathway No. 174 | 100.004
(uM^-1 s^-1) | 100
(s^-1) | Kd(bf) = 1(uM) | - | Substrate:
CaMKII-CaMCa3
Ca
Products:
CaMKII-CaMCa4
|
| 12 | CaMKII-bind-CaMC
a4 | CaMKII
Pathway No. 174 | 100.004
(uM^-1 s^-1) | 4.5
(s^-1) | Kd(bf) = 0.045(uM) | - | Substrate:
CaMCa4
CaMKII
Products:
CaMKII-CaMCa4
|
| 13 | CaMKII-CaM-bind-
Ca | CaMKII
Pathway No. 174 | 4
(uM^-1 s^-1) | 20
(s^-1) | Kd(bf) = 5(uM) | - | Substrate:
CaMKII-CaM
Ca
Products:
CAMKII-CaMCa
|
| 14 | Dephosph_
trapped_T286 | CaMKII
Pathway No. 174 | 0.003
(s^-1) | 0
(s^-1) | - | - | Substrate:
Trapped-T286
Products:
CaMKII-CaMCa4
|
| This rate is for the back reaction for the step represented here as a separate enzyme, but by Holmes as a single reversible reaction. Here it is treated as separate because the forward reaction is dependent on activity levels of the CaMKII, whereas the back reaction is not. Holmes tries a range of values for this back-reaction and says that it doesn't matter much. | | 15 | dephosph_T286 | CaMKII
Pathway No. 174 | 0.003
(s^-1) | 0
(s^-1) | - | - | Substrate:
Autonomous
Products:
CaMKII
|
| 16 | Autonomous_
lose_CaMCa4 | CaMKII
Pathway No. 174 | 0.02
(s^-1) | 0
(uM^-1 s^-1) | - | - | Substrate:
Trapped-T286
Products:
Autonomous
CaMCa4
|
| Holmes says that this rate is variable, and depends on Ca conc. It is 0.1 when [Ca] = 10 nM, and 0.00355/sec when [Ca] is 1.0 For our purposes, we assume a good steady approx to this rate is 0.02. | | 17 | Autonomous-bind-
CaMCa4 | CaMKII
Pathway No. 174 | 33.2998
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate:
CaMCa4
Autonomous
Products:
Trapped-T286
|
| 18 | dephosph_T305 | CaMKII
Pathway No. 174 | 0.01
(s^-1) | 0
(s^-1) | - | - | Substrate:
Capped
Products:
Autonomous
|
| This is treated as a separate reaction because it is independent of the CaMKII concentration or activation state, unlike the forward reaction in the Holmes paper. Holmes tries a range of rates for this step, and says it doesn't matter much. | | 19 | CaN-bind-CaM | CaN
Pathway No. 175 | 0.2
(uM^-1 s^-1) | 5000
(s^-1) | Kd(bf) = 25000(uM) | - | Substrate:
CaM
CaN
Products:
CaN-CaM
|
| 20 | CaN-bind-CaMCa | CaN
Pathway No. 175 | 5
(uM^-1 s^-1) | 3750
(s^-1) | Kd(bf) = 749.9955(uM) | - | Substrate:
CaMCa
CaN
Products:
CaN-CaMCa
|
and 
color.
indicates that ordering is done according to ascending or descending order.