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Molecule Parameter List for GAP*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
GAP* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000110

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • mkp1_feedback_
    effects
    		• 4Network
    Shared_Object_mkp1_feedback_effects,  Sos,  PKC,  
    MAPK,  PLA2,  Ras,  
    PDGFR 
    This is a network involving the MAPK-PKC feedback loop with input from the PDGFR in the synapse. The distinctive feature of this model is that it includes MKP-1 induction by MAPK, and the consequent inhibitory regulation of MAPK and the feedback loop. Lots of interesting dynamics arise from this. This link provides supplementary material for the paper Bhalla US et al. Science (2002) 297(5583):1018-23. In the form of several example simulations and demos for the figures in the paper.

    GAP* acting as a Molecule in      mkp1_feedback_effects Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    GAP*
  • mkp1_feedback_
    effects
    Accession No. : 4
    		• Ras
    Pathway No. : 37    		01000No
    Phosphorylated and inactive GAP. See Boguski and McCormick 1993 Nature 366:643-654 for a review.

    GAP* acting as a Product of an Enzyme in      mkp1_feedback_effects Network
    Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PKC-active  /
    PKC-inact-GAP
  • mkp1_feedback_
    effects
    Accession No. : 4
  • Shared_Object_
    mkp1_feedback_
    effects
    Pathway No. : 32
    		• 66.6667254explicit E-S complexSubstrate
    GAP

    Product
    GAP*
    Rate constants are generic PKC rates. This reaction inactivates GAP. The reaction is from Boguski MS and McCormick F (1993) Nature 366(6456):643-54. The phosphorylation Vmax is 6x higher to account for balance of GDP-Ras:GDP-Ras.

    GAP* acting as a Substrate in a reaction in      mkp1_feedback_effects Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    dephosph-GAP
  • mkp1_feedback_
    effects
    Accession No. : 4
    		• Ras
    Pathway No. : 37    		0.1
    (s^-1)    		0
    (s^-1)    		--Substrate
    GAP*

    Product
    GAP
    Assume a reasonably good rate for dephosphorylating it, 0.1/sec. This fits well with resting levels of active kinase and the degree of activation as well as time-course of turnoff of Ras activation, but data is quite indirect.


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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