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Molecule List for Accession CaMKII_2003 (Accession Number49)

Default ordering is done according to Pathway Number. Table headers can be used for changing the default ordering.

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The entries are grouped according to Pathway Number and are alternately color coded using

and

color.

	Name	Pathway Name / Pathway No.	Accession Type	Initial Conc. (uM)	Volume (fL)	Buffered	Sum Total Of
1	CaM-Ca4	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	-
2	PP1-active	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	1.8	1000	No	-
	Cohen et al Meth Enz 159 390-408 is main source of info conc = 1.8 uM
3	CaM-Ca3	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	-
4	CaM-TR2-Ca2	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	-
	This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
5	PP2A	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.12	1000	No	-
6	CaNAB-Ca4	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	-
7	PKC-active	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.09	1000	Yes	-
8	PKA-active	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.015	1000	Yes	-
9	Ca	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.08	1000	Yes	-
10	CaM(Ca)n-CaNAB	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	CaMCa4-CaNAB CaMCa3-CaNAB CaMCa2-CANAB
11	CaNAB	PP2B Pathway No. 205	Network	1	1000	No	-
	We assume that the A and B subunits of PP2B are always bound under physiol conditions. Up to 1% of brain protein = 25 uM. I need to work out how it is distributed between cytosolic and particulate fractions. Tallant and Cheung '83 Biochem 22 3630-3635 have conc in many species, average for mammalian brain is around 1 uM.
12	CaNAB-Ca2	PP2B Pathway No. 205	Network	0	1000	No	-
13	CaMCa3-CaNAB	PP2B Pathway No. 205	Network	0	1000	No	-
14	CaMCa2-CANAB	PP2B Pathway No. 205	Network	0	1000	No	-
15	CaMCa4-CaNAB	PP2B Pathway No. 205	Network	0	1000	No	-
16	I1	PP1 Pathway No. 204	Network	1.8	1000	No	-
	I1 is a 'mixed' inhibitor, but at high enz concs it looks like a non-compet inhibitor (Foulkes et al Eur J Biochem 132 309-313 9183). We treat it as non-compet, so it just turns the enz off without interacting with the binding site. Cohen et al ann rev bioch refer to results where conc is 1.5 to 1.8 uM. In order to get complete inhib of PP1, which is at 1.8 uM, we need >= 1.8 uM.
17	I1*	PP1 Pathway No. 204	Network	0.001	1000	No	-
	Dephosph is mainly by PP2B
18	PP1-I1*	PP1 Pathway No. 204	Network	0	1000	No	-
19	PP1-I1	PP1 Pathway No. 204	Network	0	1000	No	-
20	CaMKII	CaMKII Pathway No. 202	Network	70	1000	No	-
	Huge conc of CaMKII. In PSD it is 20-40% of protein, so we assume it is around 2.5% of protein in spine as a whole. This level is so high it is unlikely to matter much if we are off a bit. This comes to about 70 uM.

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