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Enzyme List for pathway Shared_Object_CaMKII_model3 (Pathway Number 263)

 Molecule Name/
Site Name 		Km (uM) kcat (1/s)Ratio
(k2/k3)		Enzyme TypeSubstrate Product
1 Enzyme Activity:
auton-286-PSD

Enzyme Molecule:
tot-auto-PSD		500.0020.54explicit E-S complexCaMKII-CaM-PSD
  • CaMKII-thr286-Ca
    M-PSD
    • 2 Enzyme Activity:
    auton-305-PSD

    Enzyme Molecule:
    tot-auto-PSD    		50064explicit E-S complex
  • CaMKII-thr286-PS
    D
    		• CaMKII***-PSD
    3 Enzyme Activity:
    auton-autoph

    Enzyme Molecule:
    tot-auto-PSD    		500.00524explicit E-S complexCaMKII-PSD
  • CaMKII-thr286-PS
    D
    • 4 Enzyme Activity:
    CaM-act-286-PSD

    Enzyme Molecule:
    tot-CaM-CaMKII-P
    SD    		319.9960.54explicit E-S complexCaMKII-CaM-PSD
  • CaMKII-thr286-Ca
    M-PSD
    • 5 Enzyme Activity:
    CaM-act-305-PSD

    Enzyme Molecule:
    tot-CaM-CaMKII-P
    SD    		32064explicit E-S complex
  • CaMKII-thr286-PS
    D
    		• CaMKII***-PSD
    6 Enzyme Activity:
    CaM_act_autoph

    Enzyme Molecule:
    tot-CaM-CaMKII-P
    SD    		320.00224explicit E-S complexCaMKII-PSD
  • CaMKII-thr286-PS
    D
    • 7 Enzyme Activity:
    Deph-thr286

    Enzyme Molecule:
    PP1-active    		5.09912.54explicit E-S complex
  • CaMKII-thr286*-C
    aM
    		• CaMKII-CaM
    8 Enzyme Activity:
    Deph-thr286

    Enzyme Molecule:
    PP1-active_PSD    		20.24explicit E-S complex
  • CaMKII-thr286-Ca
    M-PSD
    		• CaMKII-CaM-PSD
    9 Enzyme Activity:
    Deph-thr286b

    Enzyme Molecule:
    PP1-active    		5.09912.54explicit E-S complexCaMKII-thr286
    		CaMKII
    10 Enzyme Activity:
    Deph-thr286b

    Enzyme Molecule:
    PP1-active_PSD    		20.24explicit E-S complex
  • CaMKII-thr286-PS
    D
    		• CaMKII-PSD
    11 Enzyme Activity:
    Deph-thr286c

    Enzyme Molecule:
    PP1-active    		5.09912.54explicit E-S complexCaMKII***
    		CaMK-thr305
    12 Enzyme Activity:
    Deph-thr286c

    Enzyme Molecule:
    PP1-active_PSD    		20.24explicit E-S complexCaMKII***-PSD
  • CaMKII-thr305-PS
    D
    • 13 Enzyme Activity:
    Deph-thr305

    Enzyme Molecule:
    PP1-active    		5.09912.54explicit E-S complexCaMKII***
    		CaMKII-thr286
    14 Enzyme Activity:
    Deph-thr305

    Enzyme Molecule:
    PP1-active_PSD    		20.24explicit E-S complexCaMKII***-PSD
  • CaMKII-thr286-PS
    D
    • 15 Enzyme Activity:
    Deph-thr305a

    Enzyme Molecule:
    PP1-active    		5.09912.54explicit E-S complexCaMK-thr305
    		CaMKII
    16 Enzyme Activity:
    Deph-thr305a

    Enzyme Molecule:
    PP1-active_PSD    		20.24explicit E-S complex
  • CaMKII-thr305-PS
    D
    		• CaMKII-PSD
    17 Enzyme Activity:
    dephosph-PP1-I*

    Enzyme Molecule:
    CaM_Ca_n-CaNAB    		4.97080.344explicit E-S complexPP1-I1*
    		PP1-I1
    18 Enzyme Activity:

    dephosph-PP1-I*_
    PSD

    Enzyme Molecule:
    CaM_Ca_n-CaNAB    		4.97070.344explicit E-S complexPP1-I1*
    		PP1-I1
    19 Enzyme Activity:
    dephosph_inhib1

    Enzyme Molecule:
    CaM_Ca_n-CaNAB    		4.97080.344explicit E-S complexI1*
    		I1
    20 Enzyme Activity:
    dephosph_
    inhib1_noCaM

    Enzyme Molecule:
    CaNAB-Ca4    		4.97080.0344explicit E-S complexI1*
    		I1
      The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034
    21 Enzyme Activity:
    dephosph_
    inhib1_noCaM_
    PSD

    Enzyme Molecule:
    CaNAB-Ca4    		4.97070.0344explicit E-S complexI1*
    		I1
      The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034
    22 Enzyme Activity:
    dephosph_
    inhib1_PSD

    Enzyme Molecule:
    CaM_Ca_n-CaNAB    		4.97070.344explicit E-S complexI1*
    		I1
    23 Enzyme Activity:
    phosph-AC2

    Enzyme Molecule:
    PKC-active    		33.333744explicit E-S complexAC2
    		AC2*
      Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4
    24 Enzyme Activity:
    phosph-PDE

    Enzyme Molecule:
    PKA-active    		7.500194explicit E-S complexcAMP-PDE
    		cAMP-PDE*
      Same rates as PKA-phosph-I1
    25 Enzyme Activity:
    PKA-phosph-I1

    Enzyme Molecule:
    PKA-active    		7.500194explicit E-S complexI1
    		I1*
      #s from Bramson et al CRC crit rev Biochem 15:2 93-124. They have a huge list of peptide substrates and I have chosen high-ish rates. These consts give too much PKA activity, so lower Vmax 1/3. Now, k1 = 3e-5, k2 = 36, k3 = 9 (still pretty fast). Also lower Km 1/3 so k1 = 1e-5 Cohen et al FEBS Lett 76:182-86 1977 say rate =30% PKA act on phosphokinase beta.
    26 Enzyme Activity:
    PKA-phosph-I1_
    PSD

    Enzyme Molecule:
    PKA-active    		7.500194explicit E-S complexI1
    		I1*
    27 Enzyme Activity:
    PP2A-dephosph-I1

    Enzyme Molecule:
    PP2A    		15.999924.1667explicit E-S complexI1*
    		I1
      PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6
    28 Enzyme Activity:

    PP2A-dephosph-I1
    _
    PSD

    Enzyme Molecule:
    PP2A    		15.999924.1667explicit E-S complexI1*
    		I1
      PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6
    29 Enzyme Activity:
    PP2A-dephosph-PP
    1-I*

    Enzyme Molecule:
    PP2A    		15.999924.1667explicit E-S complexPP1-I1*
    		PP1-I1
      k1 changed from 3.3e-6 to 6.6e-6
    30 Enzyme Activity:

    PP2A-dephosph-PP
    1-I*_
    PSD

    Enzyme Molecule:
    PP2A    		15.999924.1667explicit E-S complexPP1-I1*
    		PP1-I1
      k1 changed from 3.3e-6 to 6.6e-6
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