 |  |  CaRegulation |
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Reaction List for pathway IP3-3K (Pathway Number 162) | | Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| | Name | Kf | Kb | Kd | tau | Substrate | Product | | 1 |
3K*-bind-CaM | kf
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.002(uM) | - | IP3_3K*
CaM-Ca4
| IP3_3K_CaM*
| | | Communi et al, EMBO J 16; 1997 phosphorylated 3kinase has 25 fold greater sensitivity to CaM binding than the non-phosphorylated enzyme (Kd of 2nM) | | 2 |
3K-bind-CaM | kf
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.052(uM) | - | IP3_3K
CaM-Ca4
| IP3_3K_CaM
| | | Communi et al, EMBO J 16; 1997 non-phosphorylated 3kinase with low sensitivity to CaM binding (Kd = 52nM) | | 3 |
3k-CaM*-off | kf
(s^-1) | 0.4148
(uM^-1 s^-1) | Kd(cb) = 0.0092(uM) | - | 3kCaM*_ip3_
cmplx
| IP4(1345)
IP3_3K_CaM*
| | | Kf = Vmax for enzyme (Communi et al, EMBO J 16(8)) Vmax is such that enzyme activity is 9 fold above basal. Kb derived from Keq value when reaction free energy = -10 kJ/mol | | 4 |
3k-CaM*-on | kf
(uM^-1 s^-1) | 180
(s^-1) | Kd(bf) = 2.24(uM) | - | IP3(145)
IP3_3K_CaM*
| 3kCaM*_ip3_
cmplx
| | | Rates from Km of enzyme Communi et al, EMBO J 16(8) | | 5 |
3k-CaM-off | kf
(s^-1) | 0.0968
(uM^-1 s^-1) | Kd(cb) = 0.0092(uM) | - | 3kCaM_ip3_cmplx
| IP4(1345)
IP3_3K_CaM
| | | Kf = Vmax for enzyme: Erneux et al, Biochem 214; 1993 Enzyme is 2-2.5 fold more active than basal enzyme. Kb derived from equilibrium conditions for dG = -10 kJ/mol | | 6 |
3k-CaM-on | kf
(uM^-1 s^-1) | 42.001
(s^-1) | Kd(bf) = 2.2401(uM) | - | IP3_3K_CaM
IP3(145)
| 3kCaM_ip3_cmplx
| | | rates from Km for enzyme: Erneux et al, Biochem 214; 1993 Enzyme is 2-2.5 fold more active than ip3-3k, but Km is doubled. |
Pathway Details Molecule List Enzyme List Reaction List
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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