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Molecule Parameter List for DAG | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | fig2_egfr | 1 | Network |
Shared_Object_fig2_egfr, PKC, PLA2,
PLCbeta, Gq, MAPK,
Ras, EGFR, Sos,
PLC_g, CaRegulation | This network was used to generate figure 2 in Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. It consists of the MAPK cascade in a feedback loop with PKC, and receives input from the EGFR in the synapse.
Demonstration script files for generating the figures in the paper, including figure 2, are available here. |
DAG acting as a Molecule in fig2_egfr Network
DAG acting as a Product of an Enzyme in fig2_egfr Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | PLC-Ca /
PLC-Ca | fig2_egfr
Accession No. : 1 | PLCbeta
Pathway No. : 4 | 19.8413 | 10 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3
| | 2 | PLC-Ca-Gq /
PLCb-Ca-Gq | fig2_egfr
Accession No. : 1 | PLCbeta
Pathway No. : 4 | 5 | 48 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3
| | 3 | Ca.PLC_g /
PIP2_hydrolysis | fig2_egfr
Accession No. : 1 | PLC_g
Pathway No. : 10 | 97.2222 | 14 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
PIP2
Product
DAG
IP3
| | 4 | Ca.PLC_g* /
PIP2_hydrolysis | fig2_egfr
Accession No. : 1 | PLC_g
Pathway No. : 10 | 19.7917 | 57 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
PIP2
Product
DAG
IP3
|
DAG acting as a Substrate in a reaction in fig2_egfr Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | PKC-act-by-DAG | fig2_egfr
Accession No. : 1 | PKC
Pathway No. : 2 | 0.008
(uM^-1 s^-1) | 8.6348
(s^-1) | Kd(bf) = 1079.377(uM) | - | Substrate
DAG
PKC-Ca
Product
PKC-Ca-DAG
| | 2 | PKC-n-DAG | fig2_egfr
Accession No. : 1 | PKC
Pathway No. : 2 | 0.0006
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 166.6667(uM) | - | Substrate
DAG
PKC-cytosolic
Product
PKC-DAG
| | 3 | DAG-Ca-PLA2-act | fig2_egfr
Accession No. : 1 | PLA2
Pathway No. : 3 | 0.003
(uM^-1 s^-1) | 4
(s^-1) | Kd(bf) = 1333.3333(uM) | - | Substrate
DAG
PLA2-Ca*
Product
DAG-Ca-PLA2*
| | 4 | Degrade-DAG | fig2_egfr
Accession No. : 1 | PLCbeta
Pathway No. : 4 | 0.15
(s^-1) | 0
(s^-1) | Not applicable** | - | Substrate
DAG
Product
PC
|
** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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