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Molecule Parameter List for PLC

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLC participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Synaptic_ Network	16	Network	Shared_Object_Synaptic_Network,  PKC,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  CaRegulation
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated. Bhalla US Biophys J. 2002 Aug;83(2):740-52 Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62

PLC acting as a Molecule in  Synaptic_Network Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLC	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	0.8	1000	No
Total PLC = 0.8 uM see Ryu et al JBC 262 (26) pp 12511 1987

PLC acting as a Substrate in a reaction in  Synaptic_Network Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Act-PLC-Ca	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	3 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.3333(uM)	-	Substrate Ca PLC Product PLC-Ca
	Affinity for Ca = 1uM without AlF, 0.1 with: from Smrcka et al science 251 pp 804-807 1991 Assigned affinity to a Kd of 0.333 to maintain detailed balance.
2	PLC-bind-Gq	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	2.52 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.3968(uM)	-	Substrate G*GTP PLC Product PLC-Gq
	this binding does not produce active PLC. This step was needed to implement the described (Smrcka et al) increase in affinity for Ca by PLC once Gq was bound. The kinetics are the same as the binding step for Ca-PLC to Gq. Kd is constrained by detailed balance.