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Molecule Parameter List for PLC-Ca

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLC-Ca participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	1	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Synaptic_ Network	16	Network	Shared_Object_Synaptic_Network,  PKC,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  CaRegulation
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated. Bhalla US Biophys J. 2002 Aug;83(2):740-52 Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62

PLC-Ca acting as a Molecule in  Synaptic_Network Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLC-Ca	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	0	1000	No
Ca-bound form of PLCbeta. This form is moderately active. Reviewed in Sternweis et al 1992 Phil Trans Roy Soc. Lond.

PLC-Ca acting as an Enzyme in  Synaptic_Network Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PLC-Ca / PLC-Ca	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	19.8413	10	4	explicit E-S complex	Substrate PIP2 Product DAG IP3
From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. Km of 20 is higher than for the Gq bound form, but Vmax is about 1/3 of the Gq form.

PLC-Ca acting as a Substrate in a reaction in  Synaptic_Network Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Act-PLC-by-Gq	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	25.2 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0397(uM)	-	Substrate G*GTP PLC-Ca Product PLC-Ca-Gq
Affinity for Gq is > 20 nM (Smrcka et al Science251 804-807 1991) so [Gq].kf = kb so 40nM * 6e5 = kb/kf = 24e3 so kf = 4.2e-5, kb =1

PLC-Ca acting as a Product in a reaction in  Synaptic_Network Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Act-PLC-Ca	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	3 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.3333(uM)	-	Substrate Ca PLC Product PLC-Ca
	Affinity for Ca = 1uM without AlF, 0.1 with: from Smrcka et al science 251 pp 804-807 1991 Assigned affinity to a Kd of 0.333 to maintain detailed balance.
2	Inact-PLC-Gq	Synaptic_ Network Accession No. : 16	PLCbeta Pathway No. : 73	0.0133 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate PLC-Ca-Gq Product G*GDP PLC-Ca
	This process is assumed to be directly caused by the inactivation of the G*GTP to G*GDP. Hence, kf = .013 /sec = 0.8/min, same as the rate for Inact-G. kb = 0 since this is irreversible. We may be interested in studying the role of PLC as a GAP. If so, the kf would be faster here than in Inact-G