|
Enter a Search String | Special character and space not allowed in the query term.
Search string should be at least 2 characters long. |
Molecule Parameter List for PLC-Gq | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color. | Statistics | Accession and Pathway Details | |
Accession Name | Accession No. | Accession Type | Pathway Link | Synaptic_ Network | 16 | Network | Shared_Object_Synaptic_Network, PKC, PLA2, PLCbeta, Gq, MAPK, Ras, EGFR, Sos, PLC_g, CaMKII, CaM, PP1, PP2B, PKA, AC, CaRegulation | This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated. Bhalla US Biophys J. 2002 Aug;83(2):740-52 Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62 |
PLC-Gq acting as a Molecule in Synaptic_Network Network
Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | PLC-Gq | Synaptic_ Network Accession No. : 16 | PLCbeta Pathway No. : 73 | 0 | 1000 | No | Gq-bound form of PLC beta. |
PLC-Gq acting as a Substrate in a reaction in Synaptic_Network Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | PLC-Gq-bind-Ca | Synaptic_ Network Accession No. : 16 | PLCbeta Pathway No. : 73 | 30 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.0333(uM) | - | Substrate Ca PLC-Gq
Product PLC-Ca-Gq
| this step has a high affinity of 0.1 uM for Ca, from Smrcka et al 1991 Science 251:804-807 so kf /kb = 1/6e4 = 1.666e-5:1. See the Act-PLC-by-Gq reaction. Raised kf to 5e-5 based on match to conc-eff curves from Smrcka et al. |
PLC-Gq acting as a Product in a reaction in Synaptic_Network Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | PLC-bind-Gq | Synaptic_ Network Accession No. : 16 | PLCbeta Pathway No. : 73 | 2.52 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.3968(uM) | - | Substrate G*GTP PLC
Product PLC-Gq
| this binding does not produce active PLC. This step was needed to implement the described (Smrcka et al) increase in affinity for Ca by PLC once Gq was bound. The kinetics are the same as the binding step for Ca-PLC to Gq. Kd is constrained by detailed balance. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
|