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Molecule Parameter List for MAPKK-ser

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

MAPKK-ser participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	2	2	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Synaptic_ Network	16	Network	Shared_Object_Synaptic_Network,  PKC,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  CaRegulation
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated. Bhalla US Biophys J. 2002 Aug;83(2):740-52 Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62

MAPKK-ser acting as a Molecule in  Synaptic_Network Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK-ser	Synaptic_ Network Accession No. : 16	MAPK Pathway No. : 75	0	1000	No
Intermediately phophorylated, assumed inactive, form of MAPKK

MAPKK-ser acting as a Substrate for an Enzyme in  Synaptic_Network Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras*  / Raf-GTP-Ras*.2	Synaptic_ Network Accession No. : 16	MAPK Pathway No. : 75	0.159091	0.105	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK*
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994.
2	PPhosphatase2A  / MAPKK-deph-ser	Synaptic_ Network Accession No. : 16	Shared_Object_ Synaptic_ Network Pathway No. : 70	15.6566	6	4.16667	explicit E-S complex	Substrate MAPKK-ser Product MAPKK
	See parent PPhostphatase2A description for rate details

MAPKK-ser acting as a Product of an Enzyme in  Synaptic_Network Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras*  / Raf-GTP-Ras*.1	Synaptic_ Network Accession No. : 16	MAPK Pathway No. : 75	0.159091	0.105	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	This enzyme activity refers to the complex of phosphorylated Raf and activated Ras. Starting point for kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994 who report a Km of 0.8 uM and Vmax of ~500 fm/min/ug for MAPKK. These parms cannot reach the observed 4X stim of MAPK. So we increase the affinity, ie, raise k1 5X to 5.5e-6 which is equivalent to a 5x reduction in Km to about 0.16.
2	PPhosphatase2A  / MAPKK-deph	Synaptic_ Network Accession No. : 16	Shared_Object_ Synaptic_ Network Pathway No. : 70	15.6566	6	4.16667	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.