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Molecule Parameter List for MAPKK-ser | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Synaptic_
Network | 16 | Network |
Shared_Object_Synaptic_Network, PKC, PLA2,
PLCbeta, Gq, MAPK,
Ras, EGFR, Sos,
PLC_g, CaMKII, CaM,
PP1, PP2B, PKA,
AC, CaRegulation | This model is an annotated version of the synaptic signaling network.
The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated.
Bhalla US Biophys J. 2002 Aug;83(2):740-52
Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62 |
MAPKK-ser acting as a Molecule in Synaptic_Network Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | MAPKK-ser | Synaptic_
Network
Accession No. : 16 | MAPK
Pathway No. : 75 | 0 | 1000 | No | | Intermediately phophorylated, assumed inactive, form of MAPKK |
MAPKK-ser acting as a Substrate for an Enzyme in Synaptic_Network Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | Raf-GTP-Ras* /
Raf-GTP-Ras*.2 | Synaptic_
Network
Accession No. : 16 | MAPK
Pathway No. : 75 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate
MAPKK-ser
Product
MAPKK*
| | | Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. | | 2 | PPhosphatase2A /
MAPKK-deph-ser | Synaptic_
Network
Accession No. : 16 | Shared_Object_
Synaptic_
Network
Pathway No. : 70 | 15.6566 | 6 | 4.16667 | explicit E-S complex | Substrate
MAPKK-ser
Product
MAPKK
| | | See parent PPhostphatase2A description for rate details |
MAPKK-ser acting as a Product of an Enzyme in Synaptic_Network Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | Raf-GTP-Ras* /
Raf-GTP-Ras*.1 | Synaptic_
Network
Accession No. : 16 | MAPK
Pathway No. : 75 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate
MAPKK
Product
MAPKK-ser
| | | This enzyme activity refers to the complex of phosphorylated Raf and activated Ras. Starting point for kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994 who report a Km of 0.8 uM and Vmax of ~500 fm/min/ug for MAPKK. These parms cannot reach the observed 4X stim of MAPK. So we increase the affinity, ie, raise k1 5X to 5.5e-6 which is equivalent to a 5x reduction in Km to about 0.16. | | 2 | PPhosphatase2A /
MAPKK-deph | Synaptic_
Network
Accession No. : 16 | Shared_Object_
Synaptic_
Network
Pathway No. : 70 | 15.6566 | 6 | 4.16667 | explicit E-S complex | Substrate
MAPKK*
Product
MAPKK-ser
| | | See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
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