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Molecule Parameter List for AC1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

AC1 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Synaptic_ Network	16	Network	Shared_Object_Synaptic_Network,  PKC,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  CaRegulation
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated. Bhalla US Biophys J. 2002 Aug;83(2):740-52 Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62

AC1 acting as a Molecule in  Synaptic_Network Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
AC1	Synaptic_ Network Accession No. : 16	AC Pathway No. : 85	0.02	1000	No
AC concentrations are tricky due to poor antibodies. I refer to an estimate from Jacobowitz, PhD Thesis, Mount Sinai School of Medicine around Pg 149 which estimates cyclase as 1/12600 of membrane protein. This gives a whole-cell conc of about 33 nM using assumptions of 2% of cell mass being membrane protein. Defer et al 2000 Am J Physiol Renal Physiol 279:F400-F416 in a good review put AC1 and AC8 (which has similar properties) as among the highly expressed form of brain cyclase. We therefore estimate its levels as a good fraction of the 33 nM, at 20 nM.

AC1 acting as a Substrate in a reaction in  Synaptic_Network Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	CaM-bind-AC1	Synaptic_ Network Accession No. : 16	AC Pathway No. : 85	49.9998 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.02(uM)	-	Substrate AC1 CaM-Ca4 Product AC1-CaM
	Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 Assume a rapid CaM binding of 1/sec.
2	Gs-bind-AC1	Synaptic_ Network Accession No. : 16	AC Pathway No. : 85	126 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0079(uM)	-	Substrate AC1 Gs-alpha Product AC1-Gs
	Half-max 8nM from Tang et al JBC266:13 8595-8603 kb/kf = 8 nM = 4800#/cell Also assume rapid binding of 1/sec.