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Molecule Parameter List for MAPK-tyr | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | mkp1_feedback_
effects | 4 | Network |
Shared_Object_mkp1_feedback_effects, Sos, PKC,
MAPK, PLA2, Ras,
PDGFR | | This is a network involving the MAPK-PKC feedback loop with input from the PDGFR in the synapse. The distinctive feature of this model is that it includes MKP-1 induction by MAPK, and the consequent inhibitory regulation of MAPK and the feedback loop. Lots of interesting dynamics arise from this. This link provides supplementary material for the paper Bhalla US et al. Science (2002) 297(5583):1018-23. In the form of several example simulations and demos for the figures in the paper. |
MAPK-tyr acting as a Molecule in mkp1_feedback_effects Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | MAPK-tyr | mkp1_feedback_
effects
Accession No. : 4 | MAPK
Pathway No. : 35 | 0 | 1000 | No | | Haystead et al FEBS Lett. 306(1) pp 17-22 show that phosphorylation is strictly sequential, first tyr185 then thr183. |
MAPK-tyr acting as a Substrate for an Enzyme in mkp1_feedback_effects Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | MAPKK* /
MAPKKthr | mkp1_feedback_
effects
Accession No. : 4 | MAPK
Pathway No. : 35 | 0.0462963 | 0.15 | 4 | explicit E-S complex | Substrate
MAPK-tyr
Product
MAPK*
| | | Rate consts same as for MAPKKtyr. | | 2 | MKP-1** /
MKP1*-tyr-deph | mkp1_feedback_
effects
Accession No. : 4 | MAPK
Pathway No. : 35 | 0.0666667 | 1 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
MAPK-tyr
Product
MAPK
| | | 3 Feb 2000. Same rates as MKP-1. | | 3 | MKP-1 /
MKP1-tyr-deph | mkp1_feedback_
effects
Accession No. : 4 | Shared_Object_
mkp1_feedback_
effects
Pathway No. : 32 | 0.0666667 | 1 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
MAPK-tyr
Product
MAPK
| | | The original kinetics from Bhalla US and Iyengar R. (1999) Science 283(5400):381-387 have now been modified to obey the k2 = 4 * k3 rule, while keeping kcat and Km fixed. The main constraining data point is the time course of MAPK dephosphorylation, which this model satisfies. See Charles CH, Sun H, Lau LF, Tonks NK. (1993) Proc Natl Acad Sci U S A. 90(11):5292-5296 and Charles CH, Abler AS, Lau LF. (1992) Oncogene 7(1):187-190. Effective Km : 67 nM kcat = 1.43 umol/min/mg. | | 4 | MKP-2 /
MKP2-tyr-deph | mkp1_feedback_
effects
Accession No. : 4 | Shared_Object_
mkp1_feedback_
effects
Pathway No. : 32 | 0.0666667 | 1 | 4 | explicit E-S complex | Substrate
MAPK-tyr
Product
MAPK
| | | 22 Apr 2001: Based on MKP1 parameters. The original kinetics have been modified to obey the k2 = 4 * k3 rule, while keeping kcat and Km fixed. The only constraining data point is the time course of MAPK dephosphorylation, which this model satisfies. The rates are treated as the same as for MKP-1, based on Chu Y, Solski PA, Khosravi-Far R, Der CJ, Kelly K. (1996) J Biol Chem. 271(11):6497-6501. |
MAPK-tyr acting as a Product of an Enzyme in mkp1_feedback_effects Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | MAPKK* /
MAPKKtyr | mkp1_feedback_
effects
Accession No. : 4 | MAPK
Pathway No. : 35 | 0.0462963 | 0.15 | 4 | explicit E-S complex | Substrate
MAPK
Product
MAPK-tyr
| | | The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, ratio of 4 to get k2=0.6. k1=0.75/46.6nM=2.7e-5 In terms of Michaelis-Menten rates, Km = 0.046, Vmax = 0.15, ratio = 4. | | 2 | MKP-1** /
MKP1*-thr-deph | mkp1_feedback_
effects
Accession No. : 4 | MAPK
Pathway No. : 35 | 0.0666667 | 1 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
MAPK*
Product
MAPK-tyr
| | | 3 Feb 2000. Same rates as MKP1 | | 3 | MKP-1 /
MKP1-thr-deph | mkp1_feedback_
effects
Accession No. : 4 | Shared_Object_
mkp1_feedback_
effects
Pathway No. : 32 | 0.0666667 | 1 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
MAPK*
Product
MAPK-tyr
| | | See MKP1-tyr-deph | | 4 | MKP-2 /
MKP2-thr-deph | mkp1_feedback_
effects
Accession No. : 4 | Shared_Object_
mkp1_feedback_
effects
Pathway No. : 32 | 0.0666667 | 1 | 4 | explicit E-S complex | Substrate
MAPK*
Product
MAPK-tyr
| | | See MKP2-tyr-deph |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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